Amplifying multiplex spatial profiling with tyramide oligonucleotides

IF 28.3 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2026-08-17 DOI:10.1038/s41592-026-03201-y

引用次数: 0

Abstract

Integrating different modalities and flexibly amplifying signals presents a major challenge for imaging-based spatial biology. We present protein and nucleic acid serial tyramide amplification (PASTA), which combines tyramide oligonucleotide conjugates with diverse assays for horseradish peroxidase readout to enable the seamless, flexible integration and amplification of multiple signals.

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利用酪酰胺寡核苷酸扩增多重空间谱。

整合不同的模式和灵活地放大信号是基于成像的空间生物学面临的主要挑战。我们提出了蛋白质和核酸序列酪酰胺扩增（PASTA），它将酪酰胺寡核苷酸偶联物与多种辣根过氧化物酶检测相结合，以实现多种信号的无缝、灵活整合和扩增。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.