[Corrigendum] Coenzyme Q10 alleviates sevoflurane‑induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons.

Abstract

Following the publication of the above article, a concerned author drew to the authors' attention that they had apparently used an inappropriate antibody in their study: In the Materials and methods section, the authors had reported the use of Abcam's recombinant antibody against plant homeodomain (PHD) finger protein 1 (a.k.a. PHF1; cat. no. ab184951) to probe for the unrelated protein, tau paired helical filaments (PHFs). The authors acknowledged that they had made a methodological error: They were intending to detect tau phosphorylation at Ser396/404, but mistakenly had used the antibody against PHD finger protein 1 (cat. no. ab184951; Abcam); as a result, the western blot results shown in Figs. 3 and  7 for protein PHF1 were invalid. This error went unnoticed since the two antibodies shared the same abbreviation (PHF1), and the banding patterns obtained with the incorrect antibody appeared similar in trend to what the authors had expected to have found, which unfortunately masked the mistake during their internal data review. The Editor instructed the authors to repeat the PHF1 western blot experiments using the correct PHF1 antibody (Tau‑pSer396/404; cat. no. 87061‑1‑RR; Proteintech Group, Inc.). The new experiments yielded reproducible and valid results, and the authors have replaced the original data relating to protein PHF1 in Figs. 3 and 7; the revised versions of these figures are shown on the next page. Note that these errors did not affect the results or the main conclusions reported in the study. All the authors approve of the publication of this corrigendum, and are grateful to the Editor of Molecular Medicine Reports for allowing them the opportunity to publish this. The authors regret their oversight in selecting the wrong antibody for these specific western blot experiments, and apologize to the readership for any inconvenience caused. [Molecular Medicine Reports 22: 445‑453, 2020; DOI: 10.3892/mmr.2020.11131].