Green-Synthesized Silver Nanoparticles Against Acinetobacter baumannii: A Systematic Review of Antibacterial Activity, Mechanisms of Action, Toxicity, and Antibiofilm Effects

IF 3.6 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2026-08-19 DOI:10.1002/mbo3.70378

Shahla Shahbazi, Kimia Rafieibuzhani, Keyghobad Ghadiri, Yadollah Bahrami, Roya Chegene Lorestani, Maryam Meskini, Samira Sabzi, Mosayeb Rostamian

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Abstract

Acinetobacter baumannii is a major multidrug-resistant nosocomial pathogen, and new antimicrobial strategies are urgently needed. Green-synthesized silver nanoparticles (AgNPs) have emerged as promising candidates because of their broad antibacterial activity and potentially improved biocompatibility. This systematic review evaluated the antibacterial effects of pure, plant-mediated AgNPs against A. baumannii isolates. PubMed, Scopus, and Web of Science were searched from database inception to October 5, 2025 according to PRISMA guidelines. Eligible studies were original English-language reports assessing pure green-synthesized AgNPs against A. baumannii. Sixty-three studies met the inclusion criteria. Green AgNPs were generally active against A. baumannii and more often showed stronger effects than the corresponding plant extracts. The most commonly proposed mechanisms were reactive oxygen species generation, membrane and cell–wall disruption, increased permeability, and damage to proteins, lipids, or DNA. Biofilm data were limited but suggested promising inhibitory potential. Toxicity findings were mixed, ranging from good biocompatibility to cytotoxicity in sensitive cell models. Plant-mediated AgNPs show credible antibacterial potential against A. baumannii, but standardized synthesis, rigorous characterization, biofilm-focused testing, and in vivo safety studies are still needed before clinical translation.

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绿色合成纳米银抗鲍曼不动杆菌：抗菌活性、作用机制、毒性和抗菌膜效应的系统综述。

鲍曼不动杆菌是一种主要的多药耐药医院病原菌，迫切需要新的抗菌策略。绿色合成的银纳米颗粒（AgNPs）因其广泛的抗菌活性和潜在的生物相容性改善而成为有希望的候选者。本系统综述评价了纯植物介导的AgNPs对鲍曼不动杆菌分离株的抗菌作用。根据PRISMA指南，检索PubMed、Scopus和Web of Science从数据库建立到2025年10月5日。合格的研究是评估纯绿色合成AgNPs对抗鲍曼不动杆菌的原始英语报告。63项研究符合纳入标准。绿色AgNPs对鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼不动鲍曼。最常见的机制是活性氧的产生、膜和细胞壁的破坏、通透性的增加以及对蛋白质、脂质或DNA的损伤。生物膜数据有限，但显示出良好的抑制潜力。毒性研究结果好坏参半，从良好的生物相容性到敏感细胞模型的细胞毒性。植物介导的AgNPs对鲍曼不动杆菌具有可靠的抗菌潜力，但在临床转化之前，仍需要标准化的合成、严格的表征、以生物膜为重点的测试和体内安全性研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.