Kinetics and characterization of extracellular vesicle release from cells infected with a clinical isolate of respiratory syncytial virus A (ON1 genotype).

IF 3.1 3区医学 Q1 IMMUNOLOGY

Medical Microbiology and Immunology Pub Date : 2026-08-17 DOI:10.1007/s00430-026-00886-y

Matteo Fracella, Valeria Manganelli, Alessandra Pierangeli, Carolina Scagnolari, Manuel Pacchiele, Eleonora Coratti, Roberta Campagna, Gloria Riitano, Federica Fratini, Agostina Longo, Maurizio Sorice, Guido Antonelli

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引用次数: 0

Abstract

Despite the recent approval of preventive measures, the disease burden of respiratory syncytial virus (RSV) infection in children and older adults remains significant. To improve understanding of RSV disease, this study characterized extracellular microvesicles (EMVs) secreted during in vitro infection with a clinical RSV-A (ON1 genotype) strain (RSV/40G-A), isolated in 2022 from a pediatric pneumonia case. The type and content of EMVs released by A549 and BEAS-2B respiratory epithelial cells infected with this clinical isolate were analyzed up to 96 h post-infection (hpi). RSV infection induced a time-dependent increase in EMV levels compared to uninfected controls. EMVs released by infected BEAS-2B and A549 cells contained increasing levels of RSV nucleocapsid protein (RSV-NP) and viral RNA with a peak at 72 hpi. These findings suggest that the kinetics of vesicular release and EMV content are modulated during RSV infection. EMV secretion by host cells may constitute a potential mechanism for intercellular communication or immune activation.

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呼吸道合胞病毒a （ON1基因型）临床分离物感染细胞的胞外囊泡释放动力学和特性

尽管最近批准了预防措施，但儿童和老年人呼吸道合胞病毒（RSV）感染的疾病负担仍然很大。为了提高对RSV疾病的认识，本研究表征了体外感染临床RSV- a （ON1基因型）菌株（RSV/40G-A）时分泌的细胞外微泡（emv），该菌株于2022年从一名儿科肺炎病例中分离出来。检测感染A549和BEAS-2B呼吸道上皮细胞在感染后96 h （hpi）释放的emv类型和含量。与未感染的对照组相比，RSV感染诱导EMV水平的时间依赖性增加。被感染的BEAS-2B和A549细胞释放的emv含有RSV核衣壳蛋白（RSV- np）和病毒RNA水平升高，在72 hpi处达到峰值。这些发现表明，在RSV感染期间，囊泡释放动力学和EMV含量受到调节。宿主细胞分泌EMV可能构成细胞间通讯或免疫激活的潜在机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Microbiology and Immunology 医学-免疫学

CiteScore

10.60

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Medical Microbiology and Immunology (MMIM) publishes key findings on all aspects of the interrelationship between infectious agents and the immune system of their hosts. The journal´s main focus is original research work on intrinsic, innate or adaptive immune responses to viral, bacterial, fungal and parasitic (protozoan and helminthic) infections and on the virulence of the respective infectious pathogens. MMIM covers basic, translational as well as clinical research in infectious diseases and infectious disease immunology. Basic research using cell cultures, organoid, and animal models are welcome, provided that the models have a clinical correlate and address a relevant medical question. The journal also considers manuscripts on the epidemiology of infectious diseases, including the emergence and epidemic spreading of pathogens and the development of resistance to anti-infective therapies, and on novel vaccines and other innovative measurements of prevention. The following categories of manuscripts will not be considered for publication in MMIM: submissions of preliminary work, of merely descriptive data sets without investigation of mechanisms or of limited global interest, manuscripts on existing or novel anti-infective compounds, which focus on pharmaceutical or pharmacological aspects of the drugs, manuscripts on existing or modified vaccines, unless they report on experimental or clinical efficacy studies or provide new immunological information on their mode of action, manuscripts on the diagnostics of infectious diseases, unless they offer a novel concept to solve a pending diagnostic problem, case reports or case series, unless they are embedded in a study that focuses on the anti-infectious immune response and/or on the virulence of a pathogen.