Antiviral activity of biogenic silver nanoparticles against HSV-1: integrating computational modeling and experimental validation.

IF 1.9 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2026-08-01 DOI:10.18502/ijm.v18i4.22201

Mahsa Soltani, Ameneh Elikaei, Parinaz Ghadam, Sona Ayadi Hassan

{"title":"Antiviral activity of biogenic silver nanoparticles against HSV-1: integrating computational modeling and experimental validation.","authors":"Mahsa Soltani, Ameneh Elikaei, Parinaz Ghadam, Sona Ayadi Hassan","doi":"10.18502/ijm.v18i4.22201","DOIUrl":null,"url":null,"abstract":"Background and objectives: This study aimed to investigate the effects of biogenic silver nanoparticles (AgNPs) on Herpes simplex virus type 1 (HSV-1), focusing on the influence of plant-derived capping agents and their metal cores on antiviral efficacy and mechanism of action.Materials and methods: Biosynthesized AgNPs using aqueous extracts of Juglans regia (J. regia) and Malva sylvestris (M. sylvestris) were characterized, and their zeta potential was assessed. Molecular docking studies were performed to illuminate the interaction between AgNPs and viral particles. The antiviral activity was evaluated using viral titer quantification and a cytotoxicity assay, both performed on Vero cells.Results: AgNPs exhibited zeta potentials of -8.87 mV and -5.49 mV for J. regia and M. sylvestris, respectively, indicating their stability and potential for interaction with viral particles. Strong binding affinities between AgNPs and viral glycoprotein D (gD) were revealed by molecular docking, which obtained negative MolDock scores. The antiviral assays demonstrated a ten-thousand-fold reduction in viral titers.Conclusion: This research underscores the critical roles of capping agents and the metallic core in modulating the antiviral efficacy of plant-derived AgNPs against HSV-1, establishing them as a promising platform for novel antiviral therapeutics.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"18 4","pages":"584-594"},"PeriodicalIF":1.9000,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13490894/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v18i4.22201","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and objectives: This study aimed to investigate the effects of biogenic silver nanoparticles (AgNPs) on Herpes simplex virus type 1 (HSV-1), focusing on the influence of plant-derived capping agents and their metal cores on antiviral efficacy and mechanism of action.

Materials and methods: Biosynthesized AgNPs using aqueous extracts of Juglans regia (J. regia) and Malva sylvestris (M. sylvestris) were characterized, and their zeta potential was assessed. Molecular docking studies were performed to illuminate the interaction between AgNPs and viral particles. The antiviral activity was evaluated using viral titer quantification and a cytotoxicity assay, both performed on Vero cells.

Results: AgNPs exhibited zeta potentials of -8.87 mV and -5.49 mV for J. regia and M. sylvestris, respectively, indicating their stability and potential for interaction with viral particles. Strong binding affinities between AgNPs and viral glycoprotein D (gD) were revealed by molecular docking, which obtained negative MolDock scores. The antiviral assays demonstrated a ten-thousand-fold reduction in viral titers.

Conclusion: This research underscores the critical roles of capping agents and the metallic core in modulating the antiviral efficacy of plant-derived AgNPs against HSV-1, establishing them as a promising platform for novel antiviral therapeutics.

查看原文本刊更多论文

生物源性纳米银对HSV-1的抗病毒活性：整合计算模型和实验验证。

背景与目的：本研究旨在探讨生物源性银纳米颗粒（AgNPs）对单纯疱疹病毒1型（HSV-1）的作用，重点研究植物源性盖帽剂及其金属芯对其抗病毒效果和作用机制的影响。材料与方法：以核桃（Juglans regia）和马来籽（Malva sylvestris）的水提物为原料合成AgNPs，并测定其zeta电位。分子对接研究阐明了AgNPs与病毒颗粒之间的相互作用。抗病毒活性是通过病毒滴度定量和细胞毒性测定来评估的，这两种方法都是在Vero细胞上进行的。结果：AgNPs对rega和M. sylvestris的zeta电位分别为-8.87 mV和-5.49 mV，表明其稳定性和与病毒颗粒相互作用的潜力。通过分子对接，发现AgNPs与病毒糖蛋白D （gD）具有较强的结合亲和力，并获得负MolDock评分。抗病毒试验显示病毒滴度降低了一万倍。结论：本研究强调了封盖剂和金属核在调节植物源性AgNPs对HSV-1的抗病毒作用中的关键作用，并为开发新型抗病毒药物奠定了良好的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.