A highly sensitive electrochemical sensor based on poly(alizarin red)/gold nanoflowers/molybdenum disulfide composite for the detection of methotrexate

IF 2 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2026-08-21 DOI:10.1007/s44211-026-00958-2

Anqing Wang, Xiaorui Jiang, Dingding Duan

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引用次数: 0

Abstract

A sensitive electrochemical sensor for the detection of methotrexate (MTX), a widely used anticancer and anti‑autoimmune drug, was constructed. MTX monitoring is of great significance for reducing toxic side effects and guaranteeing clinical medication safety. The sensor was based on a glassy carbon electrode (GCE) modified with a nanocomposite of molybdenum disulfide (MoS₂) nanosheets loaded with gold gold nanoflowers (AuNFs), followed by the electrochemical polymerization of alizarin red (AR) to form a stable polymeric film (PAR) on its surface. The MoS₂ nanosheets were prepared via liquid-phase exfoliation, and the AuNPs were deposited onto them via electrodeposition. The morphology of the modified electrode (PAR/Au@MoS₂/GCE) was characterized by scanning electron microscopy (SEM), and its electrochemical properties were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The synergistic effect of the nanocomposite and the conductive polymer significantly enhanced the electrocatalytic activity towards MTX oxidation. Under optimized conditions, the oxidation peak current of MTX at the modified electrode was nearly six times higher than that at a bare GCE. The sensor exhibited a linear response to MTX concentrations ranging from 0.4 to 38.6 µM, with a low detection limit of 0.027µM (S/N = 3). The sensor demonstrated excellent reproducibility, stability, and selectivity. Its practical applicability was successfully validated by determining MTX content in commercial tablet formulations with satisfactory recoveries.

Graphical abstract

A PAR/Au@MoS₂/GCE electrochemical sensor was fabricated via sonication, electrodeposition, and electropolymerization for the sensitive detection of methotrexate.

查看原文本刊更多论文

基于聚茜素红/金纳米花/二硫化钼复合材料的高灵敏度电化学传感器检测甲氨蝶呤。

建立了一种用于检测广泛应用的抗癌和抗自身免疫药物甲氨蝶呤（MTX）的灵敏电化学传感器。MTX监测对减少毒副作用，保证临床用药安全具有重要意义。该传感器基于一种玻璃碳电极（GCE），该电极用二硫化钼（MoS2）纳米复合材料修饰，负载金纳米花（AuNFs），然后在其表面电化学聚合芫花红（AR），形成稳定的聚合物膜（PAR）。通过液相剥离法制备二硫化钼纳米片，并通过电沉积将AuNPs沉积在纳米片上。利用扫描电镜（SEM）表征了改性电极（PAR/Au@MoS2/GCE）的形貌，并利用循环伏安法（CV）和电化学阻抗谱（EIS）研究了改性电极的电化学性能。纳米复合材料与导电聚合物的协同作用显著提高了MTX氧化的电催化活性。在优化条件下，MTX在修饰电极上的氧化峰电流比在裸GCE下的氧化峰电流高近6倍。该传感器对MTX浓度在0.4 ~ 38.6µM范围内呈线性响应，低检出限为0.027µM （S/N = 3）。该传感器具有良好的重现性、稳定性和选择性。通过对商业片剂中MTX含量的测定，验证了该方法的实用性，回收率满意。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.