Buttiauxella cochleicola sp. nov., a novel coliform bacterium with β-d-glucuronidase activity, isolated from gastropods and drinking water.

IF 2.3 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2026-08-01 DOI:10.1099/ijsem.0.007265

Michael Hügler, Carolin Leister, Vera Aschmann, Monika Bösl, Lydia Grabner, Luisa Kurcz, Sarah Kirstein, Jacqueline Wolf, Richard L Hahnke, Vera Thiel

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Abstract

Four strains of coliform bacteria were isolated from drinking water (strains A2-C1_F and A2-C2_NF) and gastropod samples (strains W03-F01^T and S04-F03). 16S rRNA gene sequence analysis showed the highest similarities to members of the genus Buttiauxella (>98%), indicating that all strains are members of this genus. Phylogenomic analyses revealed that strains W03-F01^T, S04-F03 and A2-C1_F can be assigned to a putative new Buttiauxella species, while strain A2-C2_NF is a member of the species Buttiauxella ferragutiae. The strains W03-F01^T, S04-F03 and A2-C1_F show fluorescence in the Colilert system, indicating unusual β-d-glucuronidase activity, typically considered indicative for Escherichia coli (and thus faecal contamination) in water quality analysis. The draft genome of strain W03-F01^T has a size of 4.79 Mb and a G+C content of 50.0 mol%. With average nucleotide identity and digital DNA-DNA hybridization values of 96.2 and 68.8%, respectively, strain W03-F01^T was most closely related to Buttiauxella massiliensis DSM 110695^T. Additional extensive physiological, phenotypic and chemotaxonomic characterization of the strains and all reference strains confirmed the proposal of a novel species. Therefore, the strains W03-F01^T, S04-F03 and A2-C1_F represent a novel species for which the name Buttiauxella cochleicola sp. nov. is proposed. The type strain is W03-F01^T (=DSM 113920^T=CIP 112567^T).

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从腹足类动物和饮用水中分离出一种具有β-d-葡萄糖醛酸酶活性的新型大肠菌群。

从饮用水和腹足类样品中分离出4株大肠菌群（菌株A2-C1_F和A2-C2_NF）和菌株W03-F01T和S04-F03)。16S rRNA基因序列分析显示，该菌株与Buttiauxella属成员的相似性最高（>98%），表明所有菌株均为该属成员。系统发育分析表明，菌株W03-F01T、S04-F03和A2-C1_F可归属于一个假定的Buttiauxella feragutiae新种，菌株A2-C2_NF为该种成员。菌株W03-F01T、S04-F03和A2-C1_F在Colilert系统中显示荧光，表明β-d-葡萄糖醛酸酶活性异常，在水质分析中通常被认为是大肠杆菌（因此是粪便污染）的指示物。菌株W03-F01T的基因组草图大小为4.79 Mb， G+C含量为50.0 mol%。菌株W03-F01T与马斯良Buttiauxella massiliensis DSM 110695T亲缘关系最密切，平均核苷酸同源性和数字DNA-DNA杂交值分别为96.2和68.8%。对菌株和所有参考菌株进行了广泛的生理、表型和化学分类鉴定，证实了这是一个新种。因此，菌株W03-F01T、S04-F03和A2-C1_F代表了一个新种，建议将其命名为Buttiauxella cochleicola sp. 11。型应变为W03-F01T （=DSM 113920T=CIP 112567T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.