Comparative evaluation of ultracentrifugation and PEG-based precipitation of mesenchymal stem cell‑derived extracellular vesicles: equivalent functionality with enhanced practicality.
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引用次数: 0
Abstract
Aim: The isolation efficiency of extracellular vesicles (EVs) is essential to ensure their purity and biological properties. Ultracentrifugation is a traditional method which utilizes high centrifugal forces to separate EVs based on their density. The polyethylene glycol (PEG)-based precipitation method is a scalable and cost-efficient alternative to ultracentrifugation. In this study, two popular techniques used for the isolation of EVs from umbilical cord tissue-derived mesenchymal stem cells (UCT-MSCs): ultracentrifugation and polyethylene glycol PEG-based precipitation methods are examined.
Materials and methods: The UCT-MSCs were cultured in a xeno-free environment using human platelet lysate (hPL) to avoid the risk of xenogenic contamination. The MSCs and EVs were characterized to confirm their physical and functional properties. Cytotoxicity was analyzed in both normal and cancer cells, and the cell migration assay was conducted on cancer cells treated with the EVs to examine the biocompatibility and migratory effects.
Results and conclusions: The similarity in physical, molecular, and functional characterization of UC-EVs and PEG-EVs suggests that PEG-based precipitation maintained the integrity and biological properties of EVs. Overall, the PEG-based precipitation method for EVs isolation is safe and efficient, making them suitable for regeneration and cancer research.
期刊介绍：
Delivering therapeutics in a way that is right for the patient - safe, painless, reliable, targeted, efficient and cost effective - is the fundamental aim of scientists working in this area. Correspondingly, this evolving field has already yielded a diversity of delivery methods, including injectors, controlled release formulations, drug eluting implants and transdermal patches. Rapid technological advances and the desire to improve the efficacy and safety profile of existing medications by specific targeting to the site of action, combined with the drive to improve patient compliance, continue to fuel rapid research progress. Furthermore, the emergence of cell-based therapeutics and biopharmaceuticals such as proteins, peptides and nucleotides presents scientists with new and exciting challenges for the application of therapeutic delivery science and technology. Successful delivery strategies increasingly rely upon collaboration across a diversity of fields, including biology, chemistry, pharmacology, nanotechnology, physiology, materials science and engineering. Therapeutic Delivery recognizes the importance of this diverse research platform and encourages the publication of articles that reflect the highly interdisciplinary nature of the field. In a highly competitive industry, Therapeutic Delivery provides the busy researcher with a forum for the rapid publication of original research and critical reviews of all the latest relevant and significant developments, and focuses on how the technological, pharmacological, clinical and physiological aspects come together to successfully deliver modern therapeutics to patients. The journal delivers this essential information in concise, at-a-glance article formats that are readily accessible to the full spectrum of therapeutic delivery researchers.
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