Clone-Specific Cytogenetic Features Revealed by a New Repetitive Marker in Dojo Loach.

IF 2 4区生物学 Q4 CELL BIOLOGY

Cytogenetic and Genome Research Pub Date : 2026-08-11 DOI:10.1159/000552480

Kiko Shibata, Masamichi Kuroda, Toshiya Nishimura, Etsuro Yamaha, Katsutoshi Arai, Takafumi Fujimoto

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引用次数: 0

Abstract

Introduction: Most fish reproduce sexually, but some species reproduce asexually through gynogenesis. Gynogenetic species are thought to provide important insight into the evolution of sexual reproduction, and investigations into their origins are required. Clonal loaches exist in certain areas of Japan and may have resulted from a past hybridization event between the two genetically divergent groups (A and B). However, the intergroup hybrids between current groups A and B show patterns different from those of clonal loaches, and comparisons distinguishing the ancestral group within clonal loaches from the current group are needed.

Methods: We used NGS data to search for repetitive sequences that could serve as markers to reveal genetic differences between species and populations and developed new cytogenetic marker, M407.

Results: The karyotypes and 18S rDNA signals were conserved across all examined individuals. M407 signals were detected in group B and in the ancestral groups A and B of clonal loaches but not in the current group A.

Conclusion: Cytogenetic results suggest that sequences associated with group B may have been introgressed or structurally incorporated into the ancestral group A genome during hybridization events, leading to the formation of clonal loaches.

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一个新的重复标记揭示了道场泥鳅克隆特异性细胞遗传学特征。

简介：大多数鱼类有性繁殖，但也有一些鱼类通过雌性生殖进行无性繁殖。雌核发生物种被认为对有性生殖的进化提供了重要的见解，对它们的起源进行调查是必要的。克隆泥鳅存在于日本的某些地区，可能是两个遗传差异群体（a和B）之间过去杂交事件的结果。但是，当前A、B类群间杂交表现出与克隆泥鳅不同的繁殖模式，需要将克隆泥鳅的祖先类群与当前类群进行比较。方法：利用NGS数据寻找可作为种、居群遗传差异标记的重复序列，开发新的细胞遗传标记M407。结果：所有检测个体的核型和18S rDNA信号都是保守的。在克隆泥鳅的B组和A、B组中检测到M407信号，但在A组中未检测到。结论：细胞遗传学结果表明，与B组相关的序列可能在杂交过程中渗入或在结构上整合到A组基因组中，导致克隆泥鳅的形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytogenetic and Genome Research 生物-细胞生物学

CiteScore

3.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.