Changes in metabolomic profile in dogs with hepatic encephalopathy secondary to congenital portosystemic shunt suggests mitochondrial homeostasis disturbances.
Ana Fernandez Gallego, Jair Marques, Dominic Kurian, Federico Diez, Richard J Mellanby, Adam G Gow, Alexander von Kriegsheim, Kiterie M E Faller
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引用次数: 0
Abstract
Objective: To identify specific metabolomic changes in dogs showing signs of hepatic encephalopathy (HE) compared to those not showing obvious neurological signs and to follow these changes following medical treatment.
Methods: In this prospective observational study, we recruited 10 client-owned dogs diagnosed with congenital portosystemic shunt (from March 2021 through October 2022). The sera of dogs with or without signs of HE at the time of diagnosis of congenital portosystemic shunt and following medical treatment for more than 4 weeks were analyzed by UPLC-MS.
Results: Dogs showing signs of HE at the time of diagnosis (n = 6) had significantly lower serum concentrations of taurine (fold change [FC], 0.44), creatine (FC, 0.32), and guanine (FC, 0.45) and higher serum concentrations of 3-hydroxybenzaldehyde (FC, 2.51) than their counterparts not showing signs of HE (4). A marked albeit nonsignificant decrease in serum stearate concentrations was also observed in the HE group (FC, 0.12). Following medical treatment, which improved neurological signs, the concentrations of these metabolites, particularly taurine, normalized to concentrations similar to the non-HE group.
Conclusions: Dogs with HE exhibit a different serum metabolomic profile compared to dogs without signs of HE. The main changes are suggesting disturbances in mitochondrial homeostasis in dogs suffering from HE.
Clinical relevance: Supporting mitochondrial function could form the basis of new therapeutic strategies in HE and merits further investigations in future studies.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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