Diagnostic Value of the Pylori DuoTect Test for Helicobacter pylori Detection in the Vietnamese Population

IF 4.6 2区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Helicobacter Pub Date : 2026-08-10 DOI:10.1111/hel.70155

Hang Dao Viet, Binh Nguyen Phuc, Thao Dang Thi, Trang Nguyen Thi Huyen, Thuong Nguyen Thu, Uyen Vu Thi Thu, Long Hoang Bao, Long Dao Van

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Abstract

Objectives

To evaluate the diagnostic accuracy in Helicobacter pylori detection using blood test with FliD and CagA antibodies (Pylori DuoTect), comparing with rapid urease test (RUT) and urease breath test (UBT) in Vietnamese patients with upper GI symptoms.

Methods

A cross-sectional study was conducted at the Institute of Gastroenterology and Hepatology in Hanoi, Vietnam on adults with upper GI symptoms, no prior treatment of peptic ulcers, and no history of H. pylori infection. Participants underwent upper GI endoscopy and three H. pylori tests, including rapid urease test (RUT), urea breath test (UBT), and Pylori DuoTect. Pylori DuoTect was considered positive if either FliD or CagA antibodies were detected. The H. pylori infection was defined as both RUT and UBT positive. Endoscopic findings were classified using the Kyoto classification score for gastritis. Diagnostic accuracy was evaluated using sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV).

Results

From December 1, 2023, to August 9, 2024, 333 patients were recruited (mean age 45.9 years, 39.9% male). The most common symptom was epigastric pain (49.5%). The prevalences of gastritis, gastric ulcers, and duodenal ulcers were 92.7%, 12.1%, and 8.1%, respectively. Pylori DuoTect was positive in 51.9% of patients (FliD: 34.8% and CagA: 66.6%). Using the gold standard, 33.6% were diagnosed with H. pylori infection. The sensitivity, specificity, PPV, and NPV of Pylori DuoTect were 83.0%, 64.0%, 54.0%, and 88.0%, respectively. Combining Pylori DuoTect and endoscopy findings (peptic ulcer or Kyoto score ≥ 2) improved the sensitivity from 0.83 to 0.88 and increased NPV.

Conclusion

Pylori DuoTect had moderate sensitivity and low specificity in the diagnosis of H. pylori infection in Vietnamese adult patients.

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幽门螺杆菌双靶试验对越南人群幽门螺杆菌的诊断价值。

目的：比较越南上消化道症状患者的快速脲酶试验（RUT）和尿素酶呼吸试验（UBT），评价血液检测FliD和CagA抗体（pylori duoect）检测幽门螺杆菌的诊断准确性。方法：在越南河内的胃肠病学和肝病研究所进行了一项横断面研究，研究对象是有上消化道症状、没有消化性溃疡治疗史、没有幽门螺杆菌感染史的成年人。参与者接受了上消化道内窥镜检查和三项幽门螺杆菌检查，包括快速脲酶试验（RUT）、尿素呼气试验（UBT）和幽门螺杆菌duoect。如果检测到FliD或CagA抗体，则认为幽门螺杆菌duoect阳性。幽门螺杆菌感染定义为RUT和UBT均阳性。内镜检查结果使用京都胃炎分级评分进行分类。通过敏感性、特异性、阳性预测值（PPV）和阴性预测值（NPV）评估诊断准确性。结果：从2023年12月1日至2024年8月9日，共招募333例患者，平均年龄45.9岁，男性39.9%。最常见的症状是胃脘痛（49.5%）。胃炎、胃溃疡和十二指肠溃疡的患病率分别为92.7%、12.1%和8.1%。51.9%的患者Pylori duoect阳性（FliD: 34.8%, CagA: 66.6%）。按照金标准，33.6%的人被诊断为幽门螺杆菌感染。Pylori duoect的敏感性为83.0%，特异性为64.0%，PPV为54.0%，NPV为88.0%。联合幽门螺杆菌检查和内镜检查结果（消化性溃疡或京都评分≥2）将敏感性从0.83提高到0.88，并增加NPV。结论：幽门螺杆菌检测对越南成人幽门螺杆菌感染的诊断敏感性中等，特异性较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Helicobacter 医学-微生物学

CiteScore

8.40

自引率

9.10%

发文量

审稿时长

2 months

期刊介绍： Helicobacter is edited by Professor David Y Graham. The editorial and peer review process is an independent process. Whenever there is a conflict of interest, the editor and editorial board will declare their interests and affiliations. Helicobacter recognises the critical role that has been established for Helicobacter pylori in peptic ulcer, gastric adenocarcinoma, and primary gastric lymphoma. As new helicobacter species are now regularly being discovered, Helicobacter covers the entire range of helicobacter research, increasing communication among the fields of gastroenterology; microbiology; vaccine development; laboratory animal science.