Early detection of pertussis related activity by wastewater surveillance of Bordetella pertussis- and Bordetella parapertussis-associated DNA concentrations in Osaka, Japan.

IF 7 2区医学 Q1 IMMUNOLOGY

Emerging Microbes & Infections Pub Date : 2026-12-01 Epub Date: 2026-08-19 DOI:10.1080/22221751.2026.2717860

Koichiro Suzuki, Daisuke Motooka, Naomi Sakon, Kazunori Tomono

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引用次数: 0

Abstract

Pertussis causes recurrent outbreaks worldwide. Clinical surveillance can be affected by healthcare-seeking behaviour, diagnostic practices, testing sensitivity, and reporting delays. This study evaluated the utility of wastewater surveillance (WWS) by quantifying Bordetella-associated IS481 and IS1001 DNA concentrations. Weekly wastewater samples were collected from four treatment plants in Osaka Prefecture, Japan, between April 2023 and July 2025, and IS481- and IS1001-associated DNA concentrations were measured using quantitative PCR. The putative B. pertussis-associated IS481 DNA concentration increased markedly from 2024 onwards. Both threshold-based onset detection and cross-correlation analysis indicated that the wastewater IS481 DNA concentration preceded reported cases by one to several months. In contrast, the B. parapertussis-associated IS1001 DNA concentration exhibited a transient peak in 2023, which aligned with contemporaneous clinical reports but remained at low levels thereafter. These findings suggest that WWS may detect increases in pertussis-related activity earlier than clinical reporting and highlight the potential of WWS as a complementary tool to clinical surveillance for monitoring respiratory infectious diseases.

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通过对日本大阪百日咳和副百日咳博德泰拉相关DNA浓度的废水监测，早期发现百日咳相关活动。

百日咳在世界范围内引起反复爆发。临床监测可能受到求医行为、诊断做法、检测敏感性和报告延误的影响。本研究通过量化博德泰拉相关的IS481和IS1001 DNA浓度来评估废水监测（WWS）的实用性。在2023年4月至2025年7月期间，每周从日本大阪府的四个处理厂收集废水样本，并使用定量PCR测量IS481和is1001相关的DNA浓度。假定的百日咳相关的IS481 DNA浓度从2024年起显著增加。基于阈值的发病检测和相互关联分析表明，废水IS481 DNA浓度比报告病例早一到几个月。相比之下，与百日咳分枝杆菌相关的IS1001 DNA浓度在2023年出现了短暂的峰值，这与同期的临床报告一致，但此后一直保持在低水平。这些发现表明，WWS可能比临床报告更早地发现百日咳相关活动的增加，并突出了WWS作为临床监测呼吸道传染病的补充工具的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY

CiteScore

26.20

自引率

2.30%

发文量

276

审稿时长

20 weeks

期刊介绍： Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.