The first complete genome sequence of iris yellow spot virus from South America and comparative analysis with isolates of different geographic origin

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2026-08-11 DOI:10.1007/s00705-026-06718-x

Rosana Blawid, Edivânio Rodrigues de Araújo, Leandro Delalibera Geremias, Dennis Knierim, Priscila Duarte Ramirez, Paolo Margaria

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Abstract

We report the first complete nucleotide sequence of an isolate of iris yellow spot virus (IYSV; Orthotospovirus iridimaculaflavi) from Brazil. Isolate Br-25 was collected in 2025 in Imbuia, Santa Catarina, from symptomatic onion scapes. The genome of IYSV-Br-25 consisted of L, M and S genomic RNAs of 8879, 4866 and 3223 nt, respectively. Comparison of the L segment nucleotide (nt) sequence showed identities ~96% to most of the available L segment sequences from IYSV isolates of different geographic origin, with the exception of isolate DSMZ PV-0528, which showed 89.0% nt identity. The M and S segment nt sequences showed highest identity to an isolate recently reported from sugarbeet in Idaho, USA. In silico-generated Hinf1 digestion profile assigned the isolate to the previously defined IYSV_BR genotype. This work provides valuable novel information on the genetic diversity of IYSV.

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南美虹膜黄斑病毒首个全基因组序列的测定及与不同地理来源分离株的比较分析。

我们报道了巴西虹膜黄斑病毒（IYSV; Orthotospovirus iridimaculaflai）分离物的首个完整核苷酸序列。分离物Br-25于2025年在圣卡塔琳娜州的Imbuia从有症状的洋葱鳞片中收集。IYSV-Br-25基因组由L、M和S基因组rna组成，分别为8879、4866和3223 nt。L片段核苷酸（nt）序列与不同地理来源IYSV分离株L片段序列的同源性为96%，但分离株DSMZ PV-0528的同源性为89.0%。M和S段nt序列与最近报道的美国爱达荷州甜菜分离株的同源性最高。硅片生成的Hinf1消化图谱将分离物定位为先前定义的IYSVBR基因型。这项工作为IYSV的遗传多样性提供了有价值的新信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.