AKT3 and α-SMA in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis as potential prognostic indicators.
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引用次数: 0
Abstract
Purpose: Oral squamous cell carcinoma (OSCC) concomitant with oral submucous fibrosis (OSCC-OSMF) is a clinico-pathologically distinct entity. Myofibroblasts (MFs) and its differentiation plays pivotal role in tumour progression via cell cycle alterations, epithelial-mesenchymal transition (EMT), hypoxia, immunomodulatory disorders, oxidative injury and epigenetic modifications.
Objective: The present study evaluated immunohistochemical expression of AKT3 and alpha-smooth muscle actin amongst, OSMF and OSCC-OSMF and correlated with related clinicopathological parameters.
Materials and methods: 70 formalin-fixed paraffin-embedded tissue samples of histopathologically confirmed cases, including 10 NOM, 30 OSMF, and 30 OSCC-OSMF, were immunohistochemically stained with AKT3 and (α-SMA) antibodies to quantify MFs and myofibroblastic cancer associated myofibroblasts (myCAFs).
Results: The percentage of α-SMA and AKT3 positive cells was statistically significant within the OSMF and OSCC-OSMF study groups (P < 0.0001). A progressive increase in cytoplasmic expression of α-SMA and AKT3 was observed from NOM to OSMF and OSCC-OSMF. A statistically significant correlation was found between the stages of OSMF and the clinical TNM stages of OSCC-OSMF.
Conclusion: Increased stromal expression of α-SMA and AKT3 is associated with disease progression in OSMF and OSCC-OSMF. This is the first study to investigate AKT-3 expression in OSMF and OSCC arising in OSMF, and these markers may serve as potential indicators of stromal activation and tumour progression, thereby warranting further longitudinal and functional investigations.
期刊介绍：
The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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