Levofloxacin-encapsulated engineered elastosomes for targeted trans-tympanic delivery: comprehensive evaluation across in vitro, microbiological, ex vivo, and in vivo models.

IF 3.1 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2026-06-01 Epub Date: 2026-08-08 DOI:10.1080/02652048.2026.2712989

Sadek Ahmed, Ali Fayez, Heba Attia, Doaa Ahmed El-Setouhy

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引用次数: 0

Abstract

Aim: This study aimed to develop and optimise Levofloxacin-Encapsulated Engineered Elastosomes (LEEE) as a non-invasive nanocarrier to improve trans-tympanic drug delivery and therapeutic efficacy in acute otitis media (AOM).

Methods: LEEE were formulated using cholesterol, surfactant, edge activator, and terpene via thin-film hydration and optimised with a 2³ factorial design. Vesicles were characterised for particle size, zeta potential, entrapment efficiency, rheology, morphology, ex-vivo permeation, confocal laser scanning microscopy, antibacterial activity, biofilm inhibition, and in-vivo histopathology.

Results: The optimised LEEE (desirability = 0.980) exhibited a mean particle size of 100.41 ± 3.39 nm, entrapment efficiency of 86.11 ± 3.41%w/w, and zeta potential of -24.75 ± 5.02 mV. Morphology revealed uniform spherical vesicles, and rheological studies confirmed pseudoplastic behaviour. The formulation showed biphasic sustained release, enhanced tympanic membrane penetration, strong antibacterial and anti-biofilm activity, and excellent biocompatibility.

Conclusions: LEEE represent a safe and effective non-invasive nanoplatform with potential to enhance drug delivery and therapeutic outcomes in AOM.

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左氧氟沙星包封工程弹性体靶向跨鼓室递送：跨体外，微生物，离体和体内模型的综合评估。

目的：开发和优化左氧氟沙星包封工程弹性体（LEEE）作为无创纳米载体，改善急性中耳炎（AOM）的跨鼓室药物传递和治疗效果。方法：以胆固醇、表面活性剂、边缘活化剂和萜烯为原料，通过薄膜水合制备LEEE，并采用2³析因设计进行优化。对囊泡进行了粒径、zeta电位、包裹效率、流变性、形态学、体外渗透、共聚焦激光扫描显微镜、抗菌活性、生物膜抑制和体内组织病理学表征。结果：优化后的LEEE平均粒径为100.41±3.39 nm，包封效率为86.11±3.41%w/w， zeta电位为-24.75±5.02 mV，理想度为0.980。形态学显示均匀的球形囊泡，流变学研究证实了假塑性行为。该制剂具有双相缓释、增强的鼓膜渗透性、较强的抗菌和抗生物膜活性、良好的生物相容性等特点。结论：LEEE是一种安全有效的非侵入性纳米平台，具有增强AOM药物传递和治疗效果的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.