Antimicrobial, antibiofilm, and molecular docking evaluation of silver nanoparticles biosynthesized from Ocimum campechianum against Staphylococcus spp.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Microbiology Pub Date : 2026-08-08 DOI:10.1007/s10123-026-00882-5

Natilene Silva Dos Santos, Nayara Andreo, Arun Kumar Jaiswal, Milena Lima Guimarães, Tania Maria Sarmento da Silva, Helinando Pequeno de Oliveira, Mateus Matiuzzi da Costa

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引用次数: 0

Abstract

Antimicrobial resistance and the overuse of antimicrobials have driven the search for innovative therapies, particularly those based on natural compounds and medicinal plant derivatives, aiming to develop new drugs. This study aimed to investigate the antimicrobial and antibiofilm activities of crude ethanolic extract of O. campechianum (CEE-OC), and commercial (AgNPs-C), non-commercial (AgNPs-NC), and Ocimum campechianum-biosynthesized (AgNPs-OC) silver nanoparticles, against 21 Staphylococcus spp. isolates. Antimicrobial activity of rosmarinic acid (RA) against six isolates was also determined. The minimum inhibitory concentration (MIC) of CEE-OC ranged from 6,250 to 781.25 µg/mL, showing the highest antimicrobial activity among the substances evaluated. AgNPs-OC exhibited MIC values approximately fourfold lower than those of CEE-OC. In contrast, AgNPs-C and AgNPs-NC showed no antimicrobial activity at the concentrations tested. CEE-OC and AgNPs-OC inhibited biofilm formation but had no effect on established biofilms. Molecular docking analysis suggested a potential interaction between RA and the NorA efflux pump protein. The results indicated that O. Campechianum extract and biosynthesized silver nanoparticles may represent promising antimicrobial alternatives, although further studies are needed to elucidate the mechanisms underlying their activity.

查看原文本刊更多论文

山茱萸生物合成银纳米颗粒抗葡萄球菌抗菌、抗生物膜及分子对接评价。

抗菌素耐药性和过度使用抗菌素促使人们寻求创新疗法，特别是基于天然化合物和药用植物衍生物的疗法，旨在开发新药。摘要本研究旨在研究camechianum粗乙醇提取物（CEE-OC）、商业（AgNPs-C）、非商业（AgNPs-NC）和camechianum生物合成银纳米颗粒（AgNPs-OC）对21株葡萄球菌的抑菌活性和抗膜活性。测定了迷迭香酸（RA）对6株菌株的抑菌活性。CEE-OC的最小抑菌浓度（MIC）为6250 ~ 781.25µg/mL，抑菌活性最高。AgNPs-OC的MIC值比CEE-OC低约4倍。相反，AgNPs-C和AgNPs-NC在测试浓度下没有抗菌活性。CEE-OC和AgNPs-OC抑制了生物膜的形成，但对已建立的生物膜没有影响。分子对接分析表明RA与NorA外排泵蛋白之间可能存在相互作用。结果表明，稻豆提取物和生物合成纳米银可能是有前途的抗菌替代品，尽管需要进一步的研究来阐明其活性机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Microbiology 生物-生物工程与应用微生物

CiteScore

5.50

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials. A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.