Multi-component microneedle drug delivery system of sanfu herbal patch for allergic asthma in rats.

IF 6.9 3区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Drug Delivery and Translational Research Pub Date : 2026-08-08 DOI:10.1007/s13346-026-02194-7

Ying Lv, Xiaoshuang Li, Yu Wang, Xinxin Qin, Ye Tang, Meng Li, Yufei Feng

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Abstract

A dissolvable microneedle system (SFP-MNs) loaded with seven principal Sanfu Herbal Patch components was developed to assess transdermal delivery feasibility and therapeutic efficacy in a rat model of allergic asthma. SFP-MNs were fabricated using a two-step centrifugation-assisted template molding method and characterized in terms of morphology, mechanical strength, skin penetration capability, and in vitro drug release behavior. An ovalbumin (OVA)-induced allergic asthma model was established in Sprague-Dawley (SD) rats to investigate the in vivo performance of the microneedles. Pulmonary interstitial fluid was continuously sampled by microdialysis following transdermal administration, and drug concentrations were quantified using UPLC-MS/MS. Pharmacodynamic responses were evaluated by measuring histamine (HIS), immunoglobulin E (IgE), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) levels using ELISA, together with histopathological examination by hematoxylin and eosin (HE) and periodic acid-Schiff (PAS) staining. A pharmacokinetic-pharmacodynamic (PK-PD) model was subsequently established to characterize the relationship between drug exposure and therapeutic response. The prepared SFP-MNs exhibited excellent mechanical properties, efficient skin penetration, and sustained drug release. In vivo, SFP-MNs achieved effective transdermal delivery of the encapsulated components and produced superior therapeutic outcomes compared with an equivalent-dose conventional Sanfu Herbal Patch gel. Specifically, high-dose SFP-MNs markedly alleviated airway inflammation, reduced inflammatory biomarker levels, and attenuated pulmonary histopathological injury in allergic asthmatic rats. These findings demonstrate that SFP-MNs represent a promising transdermal drug delivery platform with the potential to replace conventional Sanfu Herbal Patch for the treatment of allergic asthma while providing sustained drug release and improved therapeutic efficacy.

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三附中药贴剂治疗大鼠变应性哮喘的多组分微针给药系统。

研究了一种可溶解微针系统（SFP-MNs），该微针系统装载了七种主要的三附中药贴剂成分，以评估经皮给药在变应性哮喘大鼠模型中的可行性和治疗效果。采用两步离心辅助模板成型的方法制备了多糖- mns，并对其形态、机械强度、皮肤穿透能力和体外药物释放行为进行了表征。建立卵清蛋白（OVA）诱导的SD大鼠过敏性哮喘模型，研究微针的体内性能。经皮给药后通过微透析连续取样肺间质液，并用UPLC-MS/MS定量药物浓度。采用ELISA法测定组胺（HIS）、免疫球蛋白E （IgE）、白细胞介素-10 （IL-10）和肿瘤坏死因子-α (TNF-α)水平，并用苏木精和伊红（HE）和周期性酸希夫（PAS）染色进行组织病理学检查，评价药效学反应。随后建立了药代动力学-药效学（PK-PD）模型来表征药物暴露与治疗反应之间的关系。制备的SFP-MNs具有优异的机械性能、高效的透皮性和持续的药物释放。在体内，与同等剂量的传统三伏草药贴剂凝胶相比，SFP-MNs实现了包被成分的有效透皮递送，并产生了更好的治疗效果。具体而言，高剂量的sftp - mns可显著减轻过敏性哮喘大鼠的气道炎症，降低炎症生物标志物水平，减轻肺组织病理学损伤。这些研究结果表明，SFP-MNs是一种很有前景的透皮给药平台，有可能取代传统的三附中药贴片治疗过敏性哮喘，同时提供持续的药物释放和改善的治疗效果。

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来源期刊

Drug Delivery and Translational Research MEDICINE, RESEARCH & EXPERIMENTALPHARMACOL-PHARMACOLOGY & PHARMACY

CiteScore

11.70

自引率

1.90%

发文量

160

期刊介绍： The journal provides a unique forum for scientific publication of high-quality research that is exclusively focused on translational aspects of drug delivery. Rationally developed, effective delivery systems can potentially affect clinical outcome in different disease conditions. Research focused on the following areas of translational drug delivery research will be considered for publication in the journal. Designing and developing novel drug delivery systems, with a focus on their application to disease conditions; Preclinical and clinical data related to drug delivery systems; Drug distribution, pharmacokinetics, clearance, with drug delivery systems as compared to traditional dosing to demonstrate beneficial outcomes Short-term and long-term biocompatibility of drug delivery systems, host response; Biomaterials with growth factors for stem-cell differentiation in regenerative medicine and tissue engineering; Image-guided drug therapy, Nanomedicine; Devices for drug delivery and drug/device combination products. In addition to original full-length papers, communications, and reviews, the journal includes editorials, reports of future meetings, research highlights, and announcements pertaining to the activities of the Controlled Release Society.