Construction and evaluation of 9311 resistance gene pyramiding lines with combined resistance to brown planthopper and rice blast.

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2026-08-06 eCollection Date: 2026-08-01 DOI:10.1007/s11032-026-01702-4

Shiyuan Cheng, Mingsong Zhou, Wenjie Luo, Yuqing He, Guanjun Gao

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引用次数: 0

Abstract

The brown planthopper (BPH, Nilaparvata lugens) and rice blast (Magnaporthe oryzae) are major constraints on rice production, causing extensive yield losses worldwide. Pyramiding multiple resistance genes is a key strategy for achieving durable and broad-spectrum protection against these pests and pathogens. We pyramided 13 resistance genes/QTLs (Bph10, Bph14, Bph15, Bph17, Pi2, Bph20, Bph21, Bph6, QBph3, QBph4, Bph18, Bph9, and Bph24) in the 9311 backgrounds through pairwise crossing, generating 30 pyramided lines, and true hybrids were identified by marker-assisted selection (MAS). Seedling-stage BPH resistance of the pyramided families was evaluated using the Standard Seedbox Screening Test (SSST), whereas leaf blast resistance of the selected lines was assessed by field evaluation of leaf blast. Most pyramided lines exhibited stronger resistance than their corresponding single-gene parental lines, and some also showed improved agronomic traits. These lines provide valuable breeding material for developing "Green Super Rice" with combined BPH and blast resistance and high, stable yield.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-026-01702-4.

查看原文本刊更多论文

抗褐飞虱和稻瘟病联合抗9311基因金字塔系的构建与评价。

褐飞虱（BPH, Nilaparvata lugens）和稻瘟病（Magnaporthe oryzae）是水稻生产的主要制约因素，在世界范围内造成广泛的产量损失。构建多重抗性基因是实现对这些害虫和病原体的持久和广谱保护的关键策略。我们在9311个背景中对13个抗性基因/ qtl （Bph10、Bph14、Bph15、Bph17、Pi2、Bph20、Bph21、Bph6、QBph3、QBph4、Bph18、Bph9和Bph24）进行了两两杂交，得到了30个金字塔系，并通过标记辅助选择（MAS）鉴定出了真杂交种。采用标准箱筛选试验（SSST）评价了金字塔家族苗期的BPH抗性，采用叶瘟病田间评价方法评价了所选品系的叶瘟病抗性。大多数金字塔系表现出比其对应的单基因亲本系更强的抗性，有些还表现出更好的农艺性状。这些品系为培育抗褐斑病、抗稻瘟病、高产稳产的“绿色超级稻”提供了宝贵的育种材料。补充资料：在线版本提供补充资料，网址为10.1007/s11032-026-01702-4。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.