Rapid inkjet resorbable plate scaffold 3D bioprinting technology.

Abstract

A new principle has been developed that is orders of magnitude superior to the all main 3D bioprinters. Its specifications are comparable to the breakthrough Roll Porous Scaffold 3D bioprinting technology (cell density ~1.5 × 10⁸ cell/mL, performance ~1.8 L/h, precision up to ~2500 DPI (~10 µm) for UV laser and submicron for Blu-ray one, but it is simpler. Substantial increase in the velocity of formation of solid organoid relative to conventional technique occurs due to the absence of pauses for the formation of scaffolds using stereolithography and extrusion. A new way based on prefabricated plates with two biodegradable lattice layers that dissolve quickly and slowly. The 1st mesh layer are designed to hold several cells, while the 2nd one act as a reinforcing base and filter, increasing the inkjet printing density with longer fill exposures for inkjet filling. The perimeter of the plates is made of incompressible material and controls the height of the layer of the formed bioobject when they are stacked.