Rapid serum proteome profiling of glycation and N-glycosylation in women with a history of gestational diabetes.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2026-07-14 DOI:10.1093/molecular-omics/aaiag020

Philip To, Shruti Kale, Sadie Partlow, Kristina Wallenius, Agneta Holmäng, Ulrika Andersson-Hall, Erik L Allman, Sonja Hess, H Sophia Chung

引用次数: 0

Abstract

Here, we developed and validated a high throughput proteomics workflow that enables combined profiling of global proteomes and post-translational modifications (PTMs) without conventional enrichment or fractionation. Using mass spectrometry-based methods, we identified PTM landscapes, particularly N-glycopeptides and non-enzymatic glycated peptides, from the same sample prepared for global proteomics. Applying this methodology to investigate longitudinal serum in women at 6 and 10 years post-gestational diabetes mellitus (GDM), we identified PTM signatures, including N-glycosylation and non-enzymatic glycation, associated with development of type 2 diabetes (T2D) and prediabetes, which were confirmed in an independent T2D cohort.

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妊娠期糖尿病患者糖基化和n -糖基化的快速血清蛋白质组学分析。

在这里，我们开发并验证了一种高通量蛋白质组学工作流程，该工作流程可以在不进行常规富集或分离的情况下对全局蛋白质组学和翻译后修饰（PTMs）进行组合分析。使用基于质谱的方法，我们从为全球蛋白质组学准备的相同样品中鉴定了PTM景观，特别是n-糖肽和非酶糖基化肽。应用该方法研究妊娠后糖尿病（GDM） 6年和10年妇女的纵向血清，我们发现了PTM特征，包括n-糖基化和非酶糖基化，与2型糖尿病（T2D）和前驱糖尿病的发展相关，这在一个独立的T2D队列中得到了证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.