Gastric microbiota composition and its association with rescue eradication outcomes in refractory Helicobacter pylori infection.

IF 6.3 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal Pub Date : 2026-08-05 DOI:10.1016/j.bj.2026.101023

Chia-Jung Kuo, Ngoc-Niem Bui, Yuan-Ming Yeh, Cheng-Yu Lin, Shih-Yu Wu, Wey-Ran Lin, Ming-Ling Chang, Cheng-Tang Chiu, Cheng-Hsun Chiu, Chih-Ho Lai

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引用次数: 0

Abstract

Background: The relationship between gastric microbiota composition, Helicobacter pylori infection, and eradication outcomes remains unclear. This study aims to explore the association of gastric microbiota composition among patients with refractory H. pylori infection with the outcomes of rescue therapy.

Material and methods: Patients who failed to respond to second- or third-line eradication therapies were prospectively enrolled. The composition of the gastric microbiota was analyzed using 16S rRNA gene sequencing prior to rescue therapy. The eradication outcomes were confirmed using the [¹³C]-urea breath test.

Results: Among 1,590 screened participants, 23 patients with refractory H. pylori infection who underwent rescue therapy based on antimicrobial susceptibility testing were enrolled. The alpha diversity did not differ between success and failure groups; however, the beta diversity analysis revealed a distinct microbial composition. The success group was enriched in Streptococcus, Neisseria, Cutibacterium, and Staphylococcus, with two significant species, Streptococcus mitis and Streptococcus cristatus. In contrast, Prevotella colorans was enriched in the failure group.

Conclusions: Distinct gastric microbiota signatures were associated with treatment outcomes in patients with refractory H. pylori infection. These findings provide insights that may facilitate the development of microbiota-based diagnostics and interventions to improve eradication success.

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胃微生物群组成及其与难治性幽门螺杆菌感染抢救根除结果的关系。

背景：胃微生物群组成、幽门螺杆菌感染和根除结果之间的关系尚不清楚。本研究旨在探讨难治性幽门螺杆菌感染患者胃微生物群组成与抢救治疗结果的关系。材料和方法：前瞻性纳入对二线或三线根除治疗无效的患者。在抢救治疗前，采用16S rRNA基因测序分析胃微生物群的组成。采用[13C]-尿素呼气试验确认根除结果。结果：在1590名筛选的参与者中，23名难治性幽门螺杆菌感染患者接受了基于抗菌药敏试验的抢救治疗。α多样性在成功组和失败组之间没有差异；然而，β多样性分析揭示了不同的微生物组成。成功组中链球菌、奈瑟氏菌、表皮菌和葡萄球菌含量丰富，其中有2个显著种，即mitis链球菌和cristatus链球菌。相比之下，失败组的彩色普雷沃氏菌丰富。结论：不同的胃微生物群特征与难治性幽门螺杆菌感染患者的治疗结果相关。这些发现提供的见解可能有助于开发基于微生物群的诊断和干预措施，以提高根除成功率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Journal Medicine-General Medicine

CiteScore

11.60

自引率

1.80%

发文量

128

审稿时长

42 days

期刊介绍： Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs. Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology. A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.