Repeatability of Foveal Sensitivity Measured Using Adaptive Optics Microperimetry.

IF 2.7 3区医学 Q2 OPHTHALMOLOGY

Translational Vision Science & Technology Pub Date : 2026-08-03 DOI:10.1167/tvst.15.8.3

Nivedhitha Govindasamy, William S Tuten, David H Brainard, Jessica I W Morgan

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引用次数: 0

Abstract

Purpose: To assess the within and between-session repeatability of foveal sensitivity measurements using adaptive optics (AO) microperimetry.

Methods: Five normal-sighted participants underwent AO microperimetry testing (550-nm circular stimulus) at the preferred retinal locus across two sessions (2 months ± 2 weeks). Two psychophysical paradigms were used: quick estimation of threshold (QUEST) and method of constant stimuli (MOCS). Each paradigm employed both 1.05-arcmin2 and 30.4-arcmin2 stimulus sizes; stimuli were not stabilized in real time. Trial-by-trial responses were fit with a logistic psychometric function to obtain thresholds at a criterion of 78% seen. Thresholds were converted to sensitivity. Statistical comparisons were performed using paired-sample t-tests.

Results: Sensitivities measured from QUEST and MOCS procedures were not significantly different (QUEST: 1.05 arcmin2, P = 0.36 for session 1 and P = 0.41 for session 2; MOCS: 30.4 arcmin2, P = 0.14 for session 1 and P = 0.12 for session 2). Data were thus combined across paradigms for tests of repeatability. Sensitivities for data split into two groups within session 1 and separately within session 2 were not significantly different (QUEST: 1.05 arcmin2, P = 0.34 for session 1 and P = 0.51 for session 2; MOCS: 30.4 arcmin2, P = 0.31 for session 1 and P = 0.27 for session 2). Overall, sensitivities across the two sessions were not significantly different (QUEST: 1.05 arcmin2, P = 0.06; MOCS: 30.4 arcmin2, P = 0.81). The coefficients of repeatability across sessions were 1.5 decibels (dB) and 0.4 dB for 1.05 arcmin2 and 30.4 arcmin2, respectively.

Conclusions: Sensitivity measurements from AO microperimetry were repeatable within and across sessions.

Translational relevance: Results of the present study will guide future longitudinal studies of disease progression in detecting sensitivity changes using AO microperimetry.

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使用自适应光学显微法测量中央凹灵敏度的重复性。

目的：评价自适应光学显微视野法测量中央凹灵敏度的内、间重复性。方法：5名视力正常的受试者分2个疗程（2个月±2周）在首选视网膜位置进行AO显微检查（550 nm圆形刺激）。实验采用两种心理物理范式：快速阈值估计法（QUEST）和恒刺激法（MOCS）。每种范式均采用1.05-arcmin2和30.4-arcmin2刺激大小；刺激不是实时稳定的。每个试验的反应用逻辑心理测量函数拟合，以获得78%标准的阈值。阈值被转换为敏感性。采用配对样本t检验进行统计学比较。结果：QUEST和MOCS程序测量的灵敏度没有显著差异（QUEST: 1.05 arcmin2，第1阶段P = 0.36，第2阶段P = 0.41; MOCS: 30.4 arcmin2，第1阶段P = 0.14，第2阶段P = 0.12）。因此，跨范式的数据被合并以测试可重复性。在会话1和会话2中分成两组的数据敏感性没有显著差异（QUEST: 1.05 arcmin2，会话1 P = 0.34，会话2 P = 0.51; MOCS: 30.4 arcmin2，会话1 P = 0.31，会话2 P = 0.27）。总体而言，两组的敏感性没有显著差异（QUEST: 1.05 arcmin2, P = 0.06; MOCS: 30.4 arcmin2, P = 0.81）。在1.05 arcmin2和30.4 arcmin2条件下，重复系数分别为1.5和0.4 dB。结论：AO显微术的灵敏度测量在疗程内和疗程间是可重复的。翻译相关性：本研究的结果将指导未来使用AO显微术检测敏感性变化的疾病进展的纵向研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.