High-Throughput Multi-Capillary Electrophoresis

IF 2.6 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2026-09-14 Epub Date: 2026-08-04 DOI:10.1002/elps.70133

Jana Drewes, Sarah Meyer, Isobel Roberts, Cleo Fanny, Philip A. Doble, Dayanne Mozaner Bordin, Janice I. McCauley

引用次数: 0

Abstract

The potential of multi-capillary electrophoresis (CE) for high-throughput analysis of legacy CE applications was investigated by optimisation of the separation of six pharmaceutical compounds on a single-capillary instrument, followed by transference of the method to an eight-capillary BioPhase 8800 system from SCIEX. Multi-CE had superior performance, allowing construction of calibration curves in a single run, and a five-fold reduction of run time per sample, improved linearity, accuracy and limits of detection. Method transfer from the single-capillary system required minor instrumental parameter adjustments using the same background electrolyte. The potential of multi-CE was shown via quantification of caffeine in a variety of energy drinks, demonstrating broad applicability where high-throughput and rapid analyses are desirable.

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高通量毛细管电泳。

通过在单毛细管仪器上对六种药物化合物的分离进行优化，然后将该方法转移到SCIEX的八毛管biphase 8800系统上，研究了多毛细管电泳（CE）在传统CE应用中高通量分析的潜力。Multi-CE具有优异的性能，可以在单次运行中构建校准曲线，每个样品的运行时间减少5倍，提高了线性度、准确性和检测限。从单毛细管系统转移方法需要使用相同的背景电解质进行微小的仪器参数调整。通过对各种能量饮料中的咖啡因进行定量分析，显示了多重ce的潜力，证明了高通量和快速分析的广泛适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.