Digital image analysis of AgNORs in endometrial hyperplasia and adenocarcinoma.

IF 1.3 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

Gaceta medica de Mexico Pub Date : 2025-01-01 DOI:10.24875/GMM.25000062

Catalina García-Vielma, Martha I Dávila-Rodríguez, Ricardo M Cerda-Flores, Elva I Cortés-Gutiérrez

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引用次数: 0

Abstract

Background: AgNORs are located on the satellites of the short arms of acrocentric chromosomes. Increased AgNORs have been observed in different types of cancer.

Objective: To analyze the number, size, and shape of AgNORs using digital imaging (DIA) in tissue from patients with endometrial cancer at different stages.

Materials and methods: Forty-two sections of paraffin blocks of endometrial tissue were analyzed: benign lesions (proliferative and secretory) and neoplastic lesions (hyperplasia and adenocarcinoma), stained with silver nitrate. Photographs were taken, the number of AgNORs was counted, and the area and contour of nuclei were measured by DIA.

Results: An increase in number (5.85 ± 4.15 to 13.55 ± 5.9), area (16.4 ± 5.7 to 29 ± 11.1), and nuclear contours (60.3 ± 4.9 to 79.2 ± 4.9) were observed, with p ≤ 0.05, ranging from benign to neoplastic lesions. Spherical shapes were more common in benign lesions (73%) and non-spherical shapes in neoplastic lesions (61.6%), with p < 0.000.

Conclusions: The AgNORs technique with DIA measurements is a simple technique that, when combined with other diagnostic methodologies, could aid in the differential diagnosis between benign and neoplastic lesions in endometrial tissue.

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子宫内膜增生和腺癌中AgNORs的数字图像分析。

背景：AgNORs位于顶心染色体短臂的卫星上。在不同类型的癌症中都观察到AgNORs的升高。目的：应用数字成像技术（DIA）分析不同阶段子宫内膜癌组织中AgNORs的数量、大小和形态。材料与方法：对子宫内膜组织石蜡块42张切片进行分析：良性病变（增生性和分泌性）和肿瘤病变（增生和腺癌），硝酸银染色。拍照，计数AgNORs数量，DIA测量核的面积和轮廓。结果：肿瘤数量（5.85±4.15）增加至13.55±5.9)，面积（16.4±5.7）增加至29±11.1)，核轮廓（60.3±4.9）增加至79.2±4.9),p≤0.05，从良性病变到肿瘤病变不等。良性病变以球形多见（73%），肿瘤病变以非球形多见（61.6%），p < 0.000。结论：AgNORs技术与DIA测量是一种简单的技术，当与其他诊断方法相结合时，可以帮助鉴别子宫内膜良性和肿瘤性病变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gaceta medica de Mexico 医学-医学：内科

CiteScore

1.00

自引率

0.00%

发文量

216

审稿时长

6-12 weeks

期刊介绍： Gaceta Médica de México México is the official scientific journal of the Academia Nacional de Medicina de México, A.C. Its goal is to contribute to health professionals by publishing the most relevant progress both in research and clinical practice. Gaceta Médica de México is a bimonthly peer reviewed journal, published both in paper and online in open access, both in Spanish and English. It has a brilliant editorial board formed by national and international experts.