{"title":"Potential health aspect of fermented Spirulina product with Lacticaseibacillus rhamnosus.","authors":"Akif Emre Kavak, Didem Balkanlı, Osman Sağdıç","doi":"10.1007/s10529-026-03770-0","DOIUrl":null,"url":null,"abstract":"<p><p>Bio-based fermented products have recently become increasingly important for both human health and sustainability due to the growing interest in healthy sustainable diets. In this study, spirulina was used as a substrate for the cultivation of Lacticaseibacillus rhamnosus 23.2 in a 3 L bioreactor and emphasized the evaluation of the potential health effects of the obtained bioactive products. The antioxidant potential of bioactive products has been extensively studied using assays like DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radicals, demonstrating its capacity to scavenge free radicals and mitigate oxidative stress. MTT and cell migration (scratch) assays reduced viability of Caco-2 cells and hindered cell migration cancer cells FS compared to unfermented spirulina (unFS) revealed that FS significantly reduced cell viability and migration in a dose-dependent manner, with the strongest effects observed at 5X dilution. In contrast, unFS showed weaker bioactivity. These findings highlight fermentation as a promising biotechnological approach to enhance the functional properties of natural compounds for cancer prevention and treatment. To summarize, the current study revealed that the potential of spirulina biomass to be a suitable media substrate for L. rhamnosus 23.2 strain. These findings will provide awareness toward milestone the health and food sector industrialization of FS products.</p>","PeriodicalId":8929,"journal":{"name":"Biotechnology Letters","volume":"48 4","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Letters","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10529-026-03770-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Bio-based fermented products have recently become increasingly important for both human health and sustainability due to the growing interest in healthy sustainable diets. In this study, spirulina was used as a substrate for the cultivation of Lacticaseibacillus rhamnosus 23.2 in a 3 L bioreactor and emphasized the evaluation of the potential health effects of the obtained bioactive products. The antioxidant potential of bioactive products has been extensively studied using assays like DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radicals, demonstrating its capacity to scavenge free radicals and mitigate oxidative stress. MTT and cell migration (scratch) assays reduced viability of Caco-2 cells and hindered cell migration cancer cells FS compared to unfermented spirulina (unFS) revealed that FS significantly reduced cell viability and migration in a dose-dependent manner, with the strongest effects observed at 5X dilution. In contrast, unFS showed weaker bioactivity. These findings highlight fermentation as a promising biotechnological approach to enhance the functional properties of natural compounds for cancer prevention and treatment. To summarize, the current study revealed that the potential of spirulina biomass to be a suitable media substrate for L. rhamnosus 23.2 strain. These findings will provide awareness toward milestone the health and food sector industrialization of FS products.
期刊介绍：
Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them.
All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included.
Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields.
The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories.
Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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