A Novel Platform for Fast and Cost-Effective Screening of Immunomodulatory Compounds

IF 3.2 3区生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS

Advanced biology Pub Date : 2026-08-04 DOI:10.1002/adbi.202500696

Suzana Lj. Popovic, Dejan D. Baskic, Marina M. Mitrovic, Sanja D. Matic, Tijana R. Markovic, Marijana S. Stanojevic Pirkovic, Mirela M. Jevtic, Ivana Z. Lazic, Danijela V. Todorovic

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Abstract

Compounds with immunomodulatory properties are increasingly recognized as valuable adjunctive therapies in pathological conditions associated with immune dysregulation. However, their discovery process is often costly and time-consuming. The aim of this study is to develop a low-cost, simple-to-perform, and reliable method for screening immunomodulatory agents. Human peripheral blood mononuclear cells (PBMNCs) are used as a model system. PBMNCs are isolated by density gradient centrifugation and treated with different immunostimulators and immunosuppressors. By measuring several analytes with a gas analyzer, it is revealed that changes in lactate levels alone can be used as an indicator of immune cell activation/suppression. This finding is further confirmed by measuring the expression of cell surface activation markers and HIF1α using flow cytometry, glucose concentration in PBMNC cultures using a gas analyzer, and levels of TNF-ά and IL-10 by ELISA. The results presented here reveal the simplicity and reliability of this innovative platform for screening immunomodulatory compounds.

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一种快速、低成本筛选免疫调节化合物的新平台

具有免疫调节特性的化合物越来越被认为是与免疫失调相关的病理条件下有价值的辅助疗法。然而，他们的发现过程往往是昂贵和耗时的。本研究的目的是开发一种低成本、操作简单、可靠的免疫调节剂筛选方法。人外周血单个核细胞（pbmnc）作为模型系统。采用密度梯度离心分离pbmnc，并用不同的免疫刺激剂和免疫抑制剂处理。通过用气体分析仪测量几种分析物，揭示了乳酸水平的变化可以单独用作免疫细胞激活/抑制的指标。通过流式细胞术检测细胞表面活化标志物和HIF1α的表达，气体分析仪检测PBMNC培养物中的葡萄糖浓度，ELISA检测TNF- α和IL-10的水平，进一步证实了这一发现。这里提出的结果揭示了这种筛选免疫调节化合物的创新平台的简单性和可靠性。

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