Changes in Paraoxonase-1 and Sialic Acid Levels due to Inflammatory and Oxidative Stress Response in Sheep Naturally Infected with Anaplasma spp.

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2026-08-01 DOI:10.1007/s11686-026-01374-2

Lale Başer, Nilgün Aydin

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Abstract

Purpose

This study aimed to investigate the molecular detection of Anaplasma spp. in sheep from the Northeastern Anatolia region of Türkiye and to evaluate the effects of infection on oxidative stress, antioxidant protection, and inflammation.

Methods

A total of 45 sheep blood samples (30 infected and 15 non-infected) were analyzed by molecular methods for Anaplasma spp. detection. Total sialic acid (TSA), paraoxonase-1 (PON-1) activity, malondialdehyde (MDA), whole blood reduced glutathione (GSH), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels were determined using biochemical assays and ELISA methods.

Results

Molecular analysis revealed a 345 bp gene fragment specific for Anaplasma spp. in infected sheep, whereas no amplification was observed in negative controls. Infected animals had significantly lower TSA levels (P < 0.001) and higher MDA (P < 0.001), TNF-α (P = 0.0225), and IL-6 levels (P = 0.0004), while PON-1 activity and GSH levels were significantly lower than controls (P < 0.001).

Conclusion

As Anaplasma spp. infection is followed by significant changes in redox and inflammatory response, clinical implementation of the tested markers could contribute to disease progression/resolution monitoring.

Graphical Abstract

查看原文本刊更多论文

绵羊自然感染无原体后炎症和氧化应激反应对氧磷酶-1和唾液酸水平的变化

目的：研究基耶省东北部安纳托利亚地区绵羊无原体的分子检测，探讨感染对绵羊氧化应激、抗氧化保护和炎症反应的影响。方法：采用分子方法对45份绵羊血液（感染羊30份，未感染羊15份）进行无原体检测。采用生化法和ELISA法检测总唾液酸（TSA）、对氧磷酶-1 （PON-1）活性、丙二醛（MDA）、全血还原性谷胱甘肽（GSH）、肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）水平。结果：分子分析显示感染羊无原体特异性基因片段345 bp，而阴性对照未见扩增。结论：由于无原体感染后氧化还原和炎症反应发生显著变化，因此临床应用所检测的标志物有助于监测疾病进展/消退。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.