Characterization and Evaluation of Justicia adhatoda L. and Its Green Synthesized Nanoparticles for Its Efficacy in Culex quinquefasciatus

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2026-08-01 DOI:10.1007/s11686-026-01357-3

Vikas Chandra Roy, Prince Leeban Johnwilmet, Rajalakshmi Anbalagan, Jayalakshmi Krishnan, Jefrillah Jebaseelan, Anand Setty Balakrishnan

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引用次数: 0

Abstract

Background

Justicia adhatoda L., commonly referred to as Vasa or Vasaka, is a significant therapeutic shrub in traditional ayurvedic practice. This study examines the therapeutic potential of leaves from J. adhatoda in treating Mosquito-borne diseases. We hypothesized that J. adhatoda extract and its green synthesized silver nanoparticles may have antibacterial and antilarvicidal effects.

Methods

The aqueous leaf extracts of J. adhatoda were employed as a reducing and capping agent for the AgNP synthesis. The synthesized AgNPs were confirmed using Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), UV-Visible Absorbance Spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR). The analysis of plant-derived compounds in the leaf extracts using methanol, ethanol, chloroform and water showed the existence of alkaloids, terpenoids, phenols, tannins and glycosides. The leaf extracts of J. adhatoda and their silver nanoparticles demonstrated significant antibacterial efficacy against S. epidermidis, a bacterium known to cause secondary infections, in the case of Lymphatic Filariasis, antioxidant activity was assessed to characterize the leaf’s phytochemical profile and larvicidal efficacy was evaluated against Cx. quinquefasciatus.

Results

Phytochemical analysis showed the presence of alkaloids, terpenoids, phenols, tannins, and glycosides across the solvents. Both the leaf extract and its AgNPs showed significant antibacterial efficacy against S.epidermidis and acted as a good Radical Scavenging agent. The synthesized silver nanoparticles exhibited a better larvicidal effect [LC₅₀ = 34.38 μg/ml] when compared to the aqueous leaf extract [LC₅₀ = 9060 μg/ml (9.06 mg/ml)].

Conclusions

The findings highlight the potential of J. adhatoda and its green-synthesised AgNPs as an eco-friendly and promising therapeutic agent in the control and management of larvicidal activity against Cx. quinquefasciatus and antibacterial efficacy against S. epidermidis. More comprehensive studies will be carried out to analyze the efficacy of J. adhatoda and its nanomaterial by evaluating the larvicidal and antibacterial activity by conducting in vitro analysis.

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松柏及其绿色合成纳米颗粒对致倦库蚊的药效鉴定与评价。

背景：美洲菊，通常被称为瓦萨或瓦萨卡，是传统阿育吠陀实践中重要的治疗灌木。本研究探讨了沙蚕叶对蚊媒疾病的治疗潜力。我们推测，青叶藻提取物及其绿色合成纳米银可能具有抗菌和杀虫作用。方法：以小檗叶水提物为还原剂和封盖剂合成AgNP。利用扫描电镜（SEM）、动态光散射（DLS）、紫外-可见吸收光谱（UV-Visible absor光度）、傅里叶变换红外光谱（FT-IR）对合成的AgNPs进行了验证。用甲醇、乙醇、氯仿和水对叶提取物中的植物源性化合物进行分析，发现存在生物碱、萜类、酚类、单宁和苷类。在淋巴丝虫病的情况下，对表皮葡萄球菌（一种已知引起继发性感染的细菌）的抗氧化活性进行了评估，以表征叶片的植物化学特征，并评估了对Cx的杀幼虫效果。quinquefasciatus。结果：植物化学分析显示溶剂中存在生物碱、萜类、酚类、单宁和苷类。叶提取物及其AgNPs对表皮葡萄球菌均有明显的抗菌作用，是一种良好的自由基清除剂。合成的银纳米颗粒杀灭幼虫的效果[LC50 = 34.38 μg/ml]优于水提液[LC50 = 9060 μg/ml (9.06 mg/ml)]。结论：该研究结果表明，紫叶藻及其绿色合成AgNPs作为一种生态友好的治疗剂，在控制和管理对Cx的杀虫活性方面具有很大的潜力。致倦库蚊及其对表皮葡萄球菌的抑菌效果。我们将通过体外分析来评价紫藻及其纳米材料的杀幼虫和抗菌活性，从而对其药效进行更全面的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.