Production and characterization of cellular and released polysaccharides by Papiliotrema laurentii strains bioreactor-cultivated on Mizithra secondary cheese whey

IF 10.9 3区环境科学与生态学 Q2 ENERGY & FUELS

Carbon Resources Conversion Pub Date : 2026-08-01 Epub Date: 2025-10-25 DOI:10.1016/j.crcon.2025.100390

Gabriel Vasilakis, Eleni Dalaka, Georgios Bekiaris, Sofia Marka, Andriana Kaliora, Ioannis Politis, Emmanouil Flemetakis, Georgios Theodorou, Seraphim Papanikolaou

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Abstract

Polysaccharides produced by Papiliotrema laurentii strains are of great interest due to their functional properties and potential applications in the food, feed, pharmaceutical, and cosmetic industries. Microbial bioconversion of agro-industrial by-products into high value-added compounds, such as polysaccharides, is of major importance within the framework of the circular economy, as it significantly reduces bioprocessing costs. The present study aimed to produce cellular (cPS) and released extracellular (EPS) polysaccharides through the biotechnological valorization of secondary cheese whey (SCW), a major by-product of cheese manufacturing. The non-extensively studied strain P. laurentii NRRL Y-2536, cultivated on pretreated SCW, produced biomass (14.8 g/L) rich in intracellular polysaccharides (>46 %, w/w), as well as released EPS (22.7 g/L – 504 mg/L/h). cPS were extracted using high-pressure homogenization and deproteinized in the presence of n-butanol–chloroform solvents. Both cPS and EPS were analyzed via FTIR, HPLC, and FRAP assay to assess their structure, composition, and antioxidant capacity. The effects of cPS and EPS on the viability of two human cancer cell lines, Caco-2 and HT29-MTX, were evaluated. The cPS of P. laurentii NRRL Y-2536 and P. laurentii NRRL YB-3594 were rich in β-glucans (>62 % of total cPS), while the EPS were composed of galacto-xylo-mannans. Notably, the cPS from P. laurentii YB-3594 exhibited the highest antioxidant activity. Both cPS isolates, along with the EPS from P. laurentii Y-2536, presented cytotoxic activity at the highest concentrations tested, suggesting their potential application as anticancer agents. In contrast, the heat-treated EPS from P. laurentii Y-2536 and the untreated EPS from P. laurentii YB-3594, which were non-cytotoxic, could be further explored as functional ingredients for food, feed, cosmetic, or biomedicine applications.

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在Mizithra次级奶酪乳清上培养的laurentipapiliotrema菌株的细胞多糖和释放多糖的生产及特性研究

laurentii Papiliotrema菌株生产的多糖由于其功能特性和在食品、饲料、制药和化妆品行业的潜在应用而引起了人们的极大兴趣。微生物将农用工业副产品转化为高附加值化合物，如多糖，在循环经济框架内具有重要意义，因为它大大降低了生物加工成本。本研究旨在通过对奶酪生产的主要副产物——二次奶酪乳清（SCW）的生物技术增值，生产细胞（cPS）和释放的细胞外（EPS）多糖。未经广泛研究的菌株P. laurentii NRRL Y-2536，在预处理的SCW上培养，产生的生物量（14.8 g/L）富含胞内多糖（> 46%, w/w），并释放EPS （22.7 g/L - 504 mg/L/h）。cPS采用高压均质萃取，在正丁醇-氯仿溶剂存在下脱蛋白。通过FTIR、HPLC和FRAP分析cPS和EPS的结构、组成和抗氧化能力。研究了cPS和EPS对Caco-2和HT29-MTX两种人癌细胞活力的影响。P. laurentii NRRL Y-2536和P. laurentii NRRL YB-3594的cPS富含β-葡聚糖（占总cPS的62%），而P. laurentii NRRL YB-3594的EPS由半乳糖-木糖甘露聚糖组成。其中，P. laurentii YB-3594的cPS抗氧化活性最高。这两种cPS分离物，以及来自P. laurentii Y-2536的EPS，在测试的最高浓度下表现出细胞毒活性，表明它们可能作为抗癌药物应用。相比之下，经热处理的P. laurentii Y-2536和未经处理的P. laurentii YB-3594的EPS无细胞毒性，可以进一步开发作为食品、饲料、化妆品或生物医药的功能成分。

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来源期刊

Carbon Resources Conversion Materials Science-Materials Science (miscellaneous)

CiteScore

9.90

自引率

11.70%

发文量

审稿时长

10 weeks

期刊介绍： Carbon Resources Conversion (CRC) publishes fundamental studies and industrial developments regarding relevant technologies aiming for the clean, efficient, value-added, and low-carbon utilization of carbon-containing resources as fuel for energy and as feedstock for materials or chemicals from, for example, fossil fuels, biomass, syngas, CO2, hydrocarbons, and organic wastes via physical, thermal, chemical, biological, and other technical methods. CRC also publishes scientific and engineering studies on resource characterization and pretreatment, carbon material innovation and production, clean technologies related to carbon resource conversion and utilization, and various process-supporting technologies, including on-line or off-line measurement and monitoring, modeling, simulations focused on safe and efficient process operation and control, and process and equipment optimization.