Lipopeptides from Bacillus subtilis GM5 Inhibit Biofilm Formation by Staphylococcus epidermidis through Regulation of Gene Expression.

IF 0.7 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2026-04-01 Epub Date: 2026-07-27 DOI:10.1007/s10517-026-06708-8

G F Lutfullina, M T Lutfullin, A M Mardanova

引用次数: 0

Abstract

We studied the effect of the total lipopeptide fraction from Bacillus subtilis GM5 on biofilm formation by the opportunistic pathogen Staphylococcus epidermidis and on the expression of genes involved in biofilm formation. Scanning electron microscopy showed that the total lipopeptide fraction of B. subtilis GM5 inhibits biofilm formation by S. epidermidis ATCC 14990, reduces bacterial adhesion to glass, and causes bacterial deformation. Gene expression analysis using RT-qPCR demonstrated that cell treatment with the total lipopeptide fraction suppressed the expression of genes involved in biofilm formation by 2.8 (sarA) and 3 times (icaA), which correlated with the inhibition of biofilm formation.

查看原文本刊更多论文

枯草芽孢杆菌GM5脂肽通过调控基因表达抑制表皮葡萄球菌形成生物膜。

我们研究了枯草芽孢杆菌GM5总脂肽对条件致病菌表皮葡萄球菌形成生物膜的影响以及对生物膜形成相关基因表达的影响。扫描电镜结果显示，枯草芽孢杆菌GM5总脂肽片段抑制表皮芽孢杆菌ATCC 14990形成生物膜，减少细菌对玻璃的粘附，引起细菌变形。RT-qPCR基因表达分析表明，总脂肽组分处理细胞后，参与生物膜形成的基因表达分别被抑制2.8倍（sarA）和3倍（icaA），这与抑制生物膜形成有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.