A Spontaneous Mutation in tfo X Impairs Bioluminescence in Photobacterium kishitanii ANT ‐2200

IF 3.1 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Microbiology Pub Date : 2026-07-25 DOI:10.1111/mmi.70104

Capucine Le Cam Ligier, Gwenola Simon, Charlotte Berthelier, Corinne Valette, Elisa Lefeuvre, Marc Garel, Laurie Casalot

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引用次数: 0

Abstract

Bioluminescence in luminous Vibrionaceae is often regulated by quorum sensing, yet several Photobacterium strains emit light independently of cell density, suggesting alternative regulatory inputs. Here, we characterized DimLum, a spontaneous, stable low‐luminescence mutant of Photobacterium kishitanii ANT‐2200. RNA‐seq showed a pronounced downregulation of the lux–rib operon, while whole‐genome sequencing identified a 20‐nucleotide deletion in tfo X predicted to truncate the C‐terminal region of TfoX. Complementation with the wild‐type tfo X gene restored lux–rib expression and bioluminescence, linking this mutation to the DimLum phenotype. The mutant also displayed a limited transcriptional response, including upregulation of a small set of genes such as a Cro/C1‐type regulator. Together, these results identify TfoX as an important component of the regulatory network supporting bioluminescence in P. kishitanii .

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tfo X的自发突变损害了kishitanii光杆菌ANT‐2200的生物发光

发光弧菌科的生物发光通常由群体感应调节，但一些光细菌菌株独立于细胞密度发光，表明有其他调节输入。在这里，我们对光杆菌kishitanii ANT‐2200的自发、稳定的低发光突变体DimLum进行了表征。RNA - seq显示了luxi - rib操纵子的明显下调，而全基因组测序发现tfo X中有20个核苷酸的缺失，预计会截断TfoX的C端区域。与野生型tfo X基因的互补恢复了luxi - rib的表达和生物发光，将这种突变与DimLum表型联系起来。突变体也表现出有限的转录反应，包括一小部分基因如Cro/C1型调节因子的上调。总之，这些结果表明TfoX是kishitanii中支持生物发光的调控网络的重要组成部分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Microbiology 生物-生化与分子生物学

CiteScore

7.20

自引率

5.60%

发文量

132

审稿时长

1.7 months

期刊介绍： Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.