Identification of OncoMir Biomarkers: Comprehensive Analysis of Fatty Acid Pathway Genes in Colorectal Cancer Progression and Their Prognostic Implications.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Indian Journal of Clinical Biochemistry Pub Date : 2026-08-01 Epub Date: 2024-08-28 DOI:10.1007/s12291-024-01261-5

Andisheh Oroujalian, Maryam Keshani, Farnoush Panahi, Mohadeseh Esmaeili, Zahra Koveitypour, Shahrzad Tabrizchi, Hamed Akbari, Mina Amiri, Maryam Esmaeili, Maryam Peymani, Farzad Seyed Forootan, Mohammad Hossein Nasr Esfahani, Kamran Ghaedi

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引用次数: 0

Abstract

MicroRNAs play a pivotal role in driving colorectal cancer (CRC) progression through their influence on gene expression. In this study, we conducted a comprehensive analysis of gene expression profiles in CRC cell lines, focusing on the genes involved in the fatty acid pathway and their associated microRNAs. Our analysis unveiled a consistent pattern of decreased expression for miR-16-5p, miR-195-5p, miR-27a-3p, and miR-497-5p across all CRC cell lines studied. Intriguingly, the targeted genes showed a contrasting trend, with increased expression levels. To reinforce our findings, we conducted a comparative analysis with The Cancer Genome Atlas database, which corroborated our results. Notably, we found a strong association between high expression levels of PPARD and VEGFA and a shorter Disease-Free Survival (DFS) in CRC patients. Conversely, elevated expression of PPARGC1A was linked to better overall survival outcomes. These findings underscore the significant impact of microRNAs on the regulation of gene expression in colorectal cancer and highlight potential biomarkers for assessing disease progression and patient outcomes.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-024-01261-5.

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OncoMir生物标志物的鉴定：结直肠癌进展中脂肪酸途径基因的综合分析及其预后意义。

MicroRNAs通过对基因表达的影响在驱动结直肠癌（CRC）进展中发挥关键作用。在本研究中，我们对CRC细胞系的基因表达谱进行了全面分析，重点关注脂肪酸通路相关基因及其相关的microrna。我们的分析揭示了在所研究的所有结直肠癌细胞系中miR-16-5p、miR-195-5p、miR-27a-3p和miR-497-5p表达降低的一致模式。有趣的是，目标基因表现出相反的趋势，表达水平增加。为了加强我们的发现，我们与癌症基因组图谱数据库进行了比较分析，这证实了我们的结果。值得注意的是，我们发现PPARD和VEGFA的高表达水平与CRC患者较短的无病生存期（DFS）之间存在很强的相关性。相反，PPARGC1A的表达升高与更好的总体生存结果有关。这些发现强调了microRNAs对结直肠癌基因表达调控的重要影响，并强调了评估疾病进展和患者预后的潜在生物标志物。补充资料：在线版本提供补充资料，网址为10.1007/s12291-024-01261-5。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Clinical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.50

自引率

4.80%

发文量

期刊介绍： The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.