Molecular characterization of passion fruit yellow spot virus, a novel potyvirus infecting passion fruit in La Réunion

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2026-07-25 DOI:10.1007/s00705-026-06700-7

Jean-Michel Lett, Murielle Hoareau, Evelyne Parvedy, Clarisse Clain, Janice Minatchy, Estelle Roux, Michel Grisoni, Pierre-Yves Teycheney, Pierre Lefeuvre

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引用次数: 0

Abstract

Passion fruit (Passiflora edulis) leaves displaying yellow spot symptoms were collected in La Réunion. Whilst viral indexing suggested single infections with potyviruses, high-throughput sequencing revealed both single and mixed infections involving cowpea aphid-borne mosaic virus (CABMV; species Potyvirus vignae) and a previously undescribed potyvirus, for which the name passion fruit yellow spot virus (PaYSV; tentative species Potyvirus passifloraflavamaculae) is proposed. Complete genome sequences (9,626 nucleotides) of two PaYSV isolates were obtained from these samples, using RNA sequencing after ribodepletion. The two isolates were highly similar (> 99.3% nucleotide identity) and shared the highest nucleotide (63.6%) and amino acid (71.7%) identities at the polyprotein level with passion fruit woodiness virus (PWV; Potyvirus passiflorae), values that are below the species demarcation thresholds. Our results provide compelling molecular and phylogenetic evidence for the presence of a novel potyvirus species infecting passion fruit in La Réunion.

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番莲果黄斑病毒是一种感染番莲果的新型病毒。

在拉西乌姆采得有黄斑症状的百香果（Passiflora edulis）叶片。虽然病毒索引显示单次感染是由马铃薯病毒引起的，但高通量测序显示单次和混合感染涉及豇豆蚜虫传播的花叶病毒（CABMV，种Potyvirus vignae）和一种先前未描述的马铃薯病毒，该病毒被命名为百香果黄斑病毒（PaYSV，暂定种Potyvirus passifloraflavamaculae）。利用去核后的RNA测序，获得了两株PaYSV分离株的全基因组序列（9626个核苷酸）。两株分离物在多蛋白水平上与百香果木化病毒（PWV; Potyvirus passiflorae）的核苷酸（63.6%）和氨基酸（71.7%）高度相似（核苷酸同源性为99.3%），均低于种界阈值。我们的研究结果提供了令人信服的分子和系统发育证据，证明存在一种新的感染西番莲果的病毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.