Isolation of Monoclonal Neutralizing Single-Domain Antibodies Against Clostridioides difficile Toxin B.

IF 1.5 4区生物学 Q4 CELL BIOLOGY

Acta Naturae Pub Date : 2026-04-01 DOI:10.32607/actanaturae.27874

I A Alekseeva, A S Ungur, I A Favorskaya, A I Tukhvatulin, D V Shcheblyakov, A I Korobkova, M E Komyakova, O L Voronina, N N Ryzhova, E I Ermolova, M S Kunda, D Yu Logunov, M M Bobrova, V V Makarov, S M Yudin, A L Gintsburg

引用次数: 0

Abstract

The Clostridioides difficile infection (CDI) is one of the most common nosocomial infections around. The key pathogenicity factors of this bacterium include the toxins A and B that cause the disease symptoms. Neutralization of these toxins is one of the promising strategies for CDI treatment. Here, we isolated a panel of single-domain antibodies to the toxin B CROPs domain. Two antibodies, TB5A7 and TB4A8, exhibiting potent neutralizing activity were modified to produce homodimeric forms. The TB5A7 and TB4A8 dimers were characterized by enhanced neutralizing activity and protected animals from a toxin B lethal challenge. The obtained antibodies may be used to develop new agents for CDI treatment.

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艰难梭菌毒素B单克隆中和性单域抗体的分离。

艰难梭菌感染（CDI）是最常见的医院感染之一。这种细菌的主要致病因子包括引起疾病症状的毒素A和毒素B。中和这些毒素是治疗CDI的一个很有前途的策略。在这里，我们分离了一组针对毒素B作物结构域的单域抗体。两种抗体TB5A7和TB4A8表现出强大的中和活性，修饰后产生同二聚体形式。TB5A7和TB4A8二聚体具有增强的中和活性和保护动物免受毒素B致死挑战的特征。所得抗体可用于开发新的CDI治疗药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Naturae 农林科学-林学

CiteScore

3.50

自引率

5.00%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Naturae is an international journal on life sciences based in Moscow, Russia. Our goal is to present scientific work and discovery in molecular biology, biochemistry, biomedical disciplines and biotechnology. These fields represent the most important priorities for the research and engineering development both in Russia and worldwide. Acta Naturae is also a periodical for those who are curious in various aspects of biotechnological business, innovations in pharmaceutical areas, intellectual property protection and social consequences of scientific progress. The journal publishes analytical industrial surveys focused on the development of different spheres of modern life science and technology. Being a radically new and totally unique journal in Russia, Acta Naturae is useful to both representatives of fundamental research and experts in applied sciences.