{"title":"Determination of the relationship between raftlin, oxidative stress levels, and vitamin D receptor gene polymorphism in diabetic patients.","authors":"Figen Guzelgul, Nihan Bozkurt, Merve Catak, Kubra Sahin, Marwa Abdelmageed, Shahab Ahmed Salih Gezh, Sadegul Savkin","doi":"10.1177/20420188261460573","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The pathogenesis of diabetes is affected by a complex interplay between numerous environmental and genetic factors. While single-nucleotide polymorphisms (SNPs) are not directly used as biomarkers of the disease, they can be considered an important guide for determining predisposition.</p><p><strong>Objective: </strong>To investigate the roles of raftlin, malondialdehyde (MDA), superoxide dismutase (SOD), and the vitamin D receptor (VDR) BsmI (rs1544410 A>G) polymorphism, in the clinical progression of type 2 diabetes mellitus (T2DM).</p><p><strong>Design: </strong>A retrospective observational case-control study.</p><p><strong>Methods: </strong>A total of 300 subjects were included in the study: 104 with T2DM, 85 with prediabetes, and 111 healthy controls. The VDR BsmI polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, while serum MDA, SOD, and raftlin were analyzed via the enzyme-linked immunosorbent assay (ELISA) method.</p><p><strong>Results: </strong>No statistically significant differences were observed in the allelic and genotype frequencies of the VDR gene BsmI polymorphism between T2DM prediabetes patients and controls (<i>p</i> = 0.97, <i>p</i> = 0.58, respectively). MDA levels were higher, while raftlin levels were lower in patients with T2DM and prediabetes compared to controls (<i>p</i> < 0.05). Within the T2DM cohort, HbA1c (<i>p</i> = 0.014), MDA (<i>p</i> = 0.001), and raftlin (<i>p</i> < 0.001) differed significantly between the BB and bb genotypes, with a positive correlation between the bb genotype and HbA1c (<i>p</i> = 0.008, <i>r</i> = 0.287). Receiver operating characteristic (ROC) analysis revealed raftlin's diagnostic potential among T2DM patients with the BB and bb genotypes, with an area under the curve (AUC) of 0.839, a specificity of 69.2%, and a sensitivity of 81.5%.</p><p><strong>Conclusion: </strong>To the best of our knowledge, this is the first study to demonstrate that raftlin exhibits significant diagnostic efficacy in individuals with the bb genotype within a clinical T2DM cohort.</p>","PeriodicalId":22998,"journal":{"name":"Therapeutic Advances in Endocrinology and Metabolism","volume":"17 ","pages":"20420188261460573"},"PeriodicalIF":7.8000,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13376486/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Therapeutic Advances in Endocrinology and Metabolism","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/20420188261460573","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The pathogenesis of diabetes is affected by a complex interplay between numerous environmental and genetic factors. While single-nucleotide polymorphisms (SNPs) are not directly used as biomarkers of the disease, they can be considered an important guide for determining predisposition.
Objective: To investigate the roles of raftlin, malondialdehyde (MDA), superoxide dismutase (SOD), and the vitamin D receptor (VDR) BsmI (rs1544410 A>G) polymorphism, in the clinical progression of type 2 diabetes mellitus (T2DM).
Design: A retrospective observational case-control study.
Methods: A total of 300 subjects were included in the study: 104 with T2DM, 85 with prediabetes, and 111 healthy controls. The VDR BsmI polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, while serum MDA, SOD, and raftlin were analyzed via the enzyme-linked immunosorbent assay (ELISA) method.
Results: No statistically significant differences were observed in the allelic and genotype frequencies of the VDR gene BsmI polymorphism between T2DM prediabetes patients and controls (p = 0.97, p = 0.58, respectively). MDA levels were higher, while raftlin levels were lower in patients with T2DM and prediabetes compared to controls (p < 0.05). Within the T2DM cohort, HbA1c (p = 0.014), MDA (p = 0.001), and raftlin (p < 0.001) differed significantly between the BB and bb genotypes, with a positive correlation between the bb genotype and HbA1c (p = 0.008, r = 0.287). Receiver operating characteristic (ROC) analysis revealed raftlin's diagnostic potential among T2DM patients with the BB and bb genotypes, with an area under the curve (AUC) of 0.839, a specificity of 69.2%, and a sensitivity of 81.5%.
Conclusion: To the best of our knowledge, this is the first study to demonstrate that raftlin exhibits significant diagnostic efficacy in individuals with the bb genotype within a clinical T2DM cohort.
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