Heterologous Systems in the Functional Characterization of Proteins from Phytopathogenic Fungi: A Systematic Review.

IF 3.2 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2026-07-24 DOI:10.1007/s12033-026-01592-z

Hermanna Vanesca Viana de Oliveira, Darília Christane Bomfim de Rezende, Joel Augusto Moura Porto, Eric Roberto Guimarães Rocha Aguiar, Diogo Pereira Silva de Novais, Irma Yuliana Mora-Ocampo, Ariana Silva Santos, Carlos Priminho Pirovani

{"title":"Heterologous Systems in the Functional Characterization of Proteins from Phytopathogenic Fungi: A Systematic Review.","authors":"Hermanna Vanesca Viana de Oliveira, Darília Christane Bomfim de Rezende, Joel Augusto Moura Porto, Eric Roberto Guimarães Rocha Aguiar, Diogo Pereira Silva de Novais, Irma Yuliana Mora-Ocampo, Ariana Silva Santos, Carlos Priminho Pirovani","doi":"10.1007/s12033-026-01592-z","DOIUrl":null,"url":null,"abstract":"<p><p>Fungal-host coevolution, intensified by climate change, is attracting greater efforts to characterize new virulence proteins to enable the protection of agricultural crops. Under PRISMA guidelines, in this systematic review we analyzed 122 experimental studies from the Scopus, PubMed, ScienceDirect, and Web of Science databases, mapping heterologous expression systems for fungal phytopathogen proteins from crops such as wheat, maize, rice, and apple, among other agricultural commodities. The results reveal the predominance of Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Nicotiana benthamiana, and filamentous fungi in structural and functional validation, both in vitro and in vivo. Although these heterologous hosts elucidate relevant pathogenicity mechanisms and immune suppression, gaps persist, such as the restricted use of filamentous fungi as models and the absence of standardized workflows for the integrated characterization of proteins across multiple hosts. By systematizing bibliometric trends and methodological approaches, this study reveals different strategies used to accelerate the analysis of fungal proteins, contributing to the advancement of molecular phytopathology and global food security.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Biotechnology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12033-026-01592-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Fungal-host coevolution, intensified by climate change, is attracting greater efforts to characterize new virulence proteins to enable the protection of agricultural crops. Under PRISMA guidelines, in this systematic review we analyzed 122 experimental studies from the Scopus, PubMed, ScienceDirect, and Web of Science databases, mapping heterologous expression systems for fungal phytopathogen proteins from crops such as wheat, maize, rice, and apple, among other agricultural commodities. The results reveal the predominance of Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, Nicotiana benthamiana, and filamentous fungi in structural and functional validation, both in vitro and in vivo. Although these heterologous hosts elucidate relevant pathogenicity mechanisms and immune suppression, gaps persist, such as the restricted use of filamentous fungi as models and the absence of standardized workflows for the integrated characterization of proteins across multiple hosts. By systematizing bibliometric trends and methodological approaches, this study reveals different strategies used to accelerate the analysis of fungal proteins, contributing to the advancement of molecular phytopathology and global food security.

查看原文本刊更多论文

植物病原真菌蛋白质功能鉴定的异源系统综述。

气候变化加剧了真菌与宿主的共同进化，这吸引了更多的努力来表征新的毒力蛋白，从而能够保护农作物。在PRISMA的指导下，在这篇系统综述中，我们分析了来自Scopus、PubMed、ScienceDirect和Web of Science数据库的122项实验研究，绘制了小麦、玉米、水稻和苹果等农作物中真菌植物病原体蛋白的异源表达系统。结果表明，大肠杆菌、酿酒酵母菌、毕赤酵母、benthamiana和丝状真菌在体外和体内的结构和功能验证中都具有优势。尽管这些异源宿主阐明了相关的致病性机制和免疫抑制，但差距仍然存在，例如限制使用丝状真菌作为模型，以及缺乏标准化的工作流程来综合表征多个宿主的蛋白质。通过系统化文献计量学趋势和方法学方法，本研究揭示了用于加速真菌蛋白分析的不同策略，为分子植物病理学和全球粮食安全的进步做出贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.