Lyubov A Rzhanova, Ekaterina V Kuzmenko, Alena A Permyakova, Andrei A Riabinin, Evgenii S Ruchko, Maria B Chernysheva, Ekaterina A Vorotelyak, Elena I Morgun
{"title":"Non-Healing Wound Model in Diabetic C57BL/6 Mice.","authors":"Lyubov A Rzhanova, Ekaterina V Kuzmenko, Alena A Permyakova, Andrei A Riabinin, Evgenii S Ruchko, Maria B Chernysheva, Ekaterina A Vorotelyak, Elena I Morgun","doi":"10.3390/mps9040104","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background</b>: This study focuses on developing a model of a non-healing wound that recapitulates the pathogenesis of the corresponding human pathology. <b>Methods</b>: A non-healing wound was modeled in mice with streptozotocin-induced diabetes. The following parameters were assessed: re-epithelialization, epidermal hypertrophy, wound contraction, relief index, angiogenesis, and granulation tissue maturation. These parameters were compared between diabetic mice and healthy controls. <b>Results</b>: The proposed model demonstrated a significant delay in regenerative processes compared to healthy animals. <b>Conclusions</b>: These findings <b>support the relevance</b> of this model to human pathology and <b>indicate that it may be applicable</b> for preclinical studies of drugs aimed at promoting wound regeneration.</p>","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":"9 4","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398050/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/mps9040104","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Background: This study focuses on developing a model of a non-healing wound that recapitulates the pathogenesis of the corresponding human pathology. Methods: A non-healing wound was modeled in mice with streptozotocin-induced diabetes. The following parameters were assessed: re-epithelialization, epidermal hypertrophy, wound contraction, relief index, angiogenesis, and granulation tissue maturation. These parameters were compared between diabetic mice and healthy controls. Results: The proposed model demonstrated a significant delay in regenerative processes compared to healthy animals. Conclusions: These findings support the relevance of this model to human pathology and indicate that it may be applicable for preclinical studies of drugs aimed at promoting wound regeneration.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
