Application of the International System for Reporting Serous Fluid Cytology (ISRSFC) and AgNOR Staining for Improved Diagnostic Accuracy in Serous Effusions.

IF 0.9 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2026-05-01

Magdi M Salih, Shatha A Alotaibi, Adil A M Yousif, Tariq E Elmissbah, Eman H Khalifa

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引用次数: 0

Abstract

Objective: Serous effusion smears categorized as suspicious for malignancy (SFM) pose significant diagnostic challenges. This study aimed to evaluate the diagnostic utility of argyrophilic nucleolar organizer region (AgNOR) quantification across International System for Reporting Serous Fluid Cytology (ISRS-FC) categories and to assess its association with increasing cytological atypia and malignancy risk.

Methods: A total of 160 serous effusion samples collected between October 2024 and June 2025 were examined using Papanicolaou and AgNOR staining. Cases were classified into negative for malignancy (NFM), atypia of undetermined significance (AUS), suspicious for malignancy (SFM), and malignant (MAL). Mean Ag-NOR counts were compared across categories using appropriate statistical analyses.

Results: Mean Ag-NOR counts (mean±SD) increased progressively from NFM (5.64±4.45) and AUS (5.98±5.15) to SFM (7.66±6.62) and MAL (8.64±7.81). One-way ANOVA demonstrated a significant difference among groups (p=0.001). Post hoc analysis confirmed significantly higher counts in SFM and MAL compared with NFM, and in MAL compared with AUS (p<0.05).

Conclusion: AgNOR quantification provides objective proliferative information that may enhance diagnostic stratification in serous effusion cytology, particularly in indeterminate categories. Further multicenter studies are required to establish standardized cut-off values before routine clinical application.

本刊更多论文

应用国际浆液细胞学报告系统（ISRSFC）和AgNOR染色提高浆液积液的诊断准确性。

目的：浆液积液涂片归类为可疑的恶性肿瘤（SFM）提出了重大的诊断挑战。本研究旨在评估国际浆液细胞学报告系统（ISRS-FC）分类中嗜银性核仁组织者区（AgNOR）定量的诊断效用，并评估其与细胞学非典型性和恶性肿瘤风险增加的关系。方法：采用Papanicolaou和AgNOR染色法对2024年10月至2025年6月采集的160例浆液标本进行检测。病例分为恶性阴性（NFM）、意义不确定异型（AUS）、可疑恶性（SFM）和恶性（MAL）。使用适当的统计分析比较不同类别的平均Ag-NOR计数。结果：平均Ag-NOR计数（Mean±SD）从NFM（5.64±4.45）和AUS（5.98±5.15）逐渐增加到SFM（7.66±6.62）和MAL（8.64±7.81）。单因素方差分析显示组间差异显著（p=0.001）。事后分析证实，SFM和MAL的计数明显高于NFM， MAL的计数明显高于AUS(结论：AgNOR定量提供了客观的增殖信息，可以增强浆液积液细胞学的诊断分层，特别是在不确定的类别中。在常规临床应用之前，需要进一步的多中心研究来建立标准化的临界值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.