The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.

IF 5.2 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Plant Methods Pub Date : 2026-07-21 DOI:10.1186/s13007-026-01563-0

Jérôme Gélinas Bélanger, Shona Hanaishi, Benjamin Péret

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引用次数: 0

Abstract

In plant synthetic biology, the emergence of Golden Gate (GG) cloning has led to the rapid development of numerous cloning kits that enable the standardized assembly of genetic modules and transcriptional units through a single, one-pot reaction. However, this rapid and somewhat uncoordinated expansion has introduced challenges, particularly regarding compatibility between cloning kits, which can hinder adoption and routine use in new laboratories. To address these recurring issues, this review provides a comprehensive overview of more than 25 well-established plant-specific GG cloning kits, along with an in-depth examination of the compatibility of their available modules, with the goal of improving interoperability across systems. To support a more integrated and forward-looking development of future GG-based efforts, we present key approaches for increasing system harmonization, including several domestication strategies, methylase-assisted hierarchical DNA assembly, and the inclusion of multiple cloning sites. As a whole, this review aims to streamline cloning workflows and reduce technical barriers for new plant synthetic biologists in pursuit of increasing the throughput of their experiments.

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植物金门工具包：植物合成生物学中的兼容性、互操作性和协调组装。

在植物合成生物学中，金门（GG）克隆的出现导致了许多克隆试剂盒的快速发展，这些试剂盒能够通过单一的一锅反应标准化地组装遗传模块和转录单位。然而，这种迅速而有些不协调的扩张带来了挑战，特别是在克隆试剂盒之间的兼容性方面，这可能阻碍在新实验室的采用和常规使用。为了解决这些反复出现的问题，本综述提供了超过25种成熟的植物特异性GG克隆试剂盒的全面概述，以及对其可用模块的兼容性的深入检查，目的是提高系统间的互操作性。为了支持未来基于gg的工作更加整合和前瞻性的发展，我们提出了增加系统协调的关键方法，包括几种驯化策略，甲基化酶辅助的分层DNA组装，以及包含多个克隆位点。总的来说，这篇综述旨在简化克隆工作流程，减少技术障碍，为新的植物合成生物学家追求提高他们的实验吞吐量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Methods 生物-植物科学

CiteScore

9.20

自引率

3.90%

发文量

121

审稿时长

2 months

期刊介绍： Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences. There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics. Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.