Polyhydroxyalkanoate synthase mutant derived from Bacillus cereus YB-4, which synthesizes lower molecular-weight polymers, exhibits higher enzyme activity.

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2026-09-25 DOI:10.1093/bbb/zbag110

Manami Ishii-Hyakutake, Unurbat Uranbileg, Yuki Miyahara, Takeharu Tsuge

引用次数: 0

Abstract

Polyhydroxyalkanoate (PHA) synthase (PhaRCYB4) from Bacillus cereus YB-4 is a useful enzyme that produces PHA with various molecular weights depending on the host. The PhaRCYB4 mutant with an amino acid replacement of alanine at position 77 with serine in the PhaCYB4 subunit, which synthesized lower molecular-weight PHA, exhibited 2-fold higher polymerization activity than that of the wild-type enzyme.

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源自蜡样芽孢杆菌的聚羟基烷酸合酶突变体YB-4合成低分子量聚合物，具有较高的酶活性。

来自蜡样芽孢杆菌YB-4的聚羟基烷酸酯合成酶（PhaRCYB4）是一种有用的酶，可根据宿主产生不同分子量的PHA。该突变体在PhaCYB4亚基第77位用丝氨酸取代丙氨酸，合成了低分子量PHA，聚合活性比野生型高2倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).