Exploring osmotolerant-PGPR consortium based rhizospheric microbiome for understanding metabolomic shifts in drought-stressed Oryza sativa L.

IF 5.1 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2026-07-01 Epub Date: 2026-07-08 DOI:10.1016/j.cpb.2026.100642

Aditya Sharma, Sourav Debnath, Piyush Pandey, Pratyoosh Shukla

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Abstract

Drought is a key limiting factor affecting rice (Oryza sativa L.) yield, necessitating sustainable strategies to enhance crop resilience under changing climatic conditions. The potential of a drought-tolerant plant growth–promoting rhizobacterial (PGPR) consortium, capable of withstanding up to 30% PEG-6000-induced osmotic stress, to alleviate drought stress in rice was investigated. Consortium inoculation significantly improved key physiological attributes of drought-stressed plants, including relative water content, proline accumulation, and total chlorophyll. Additionally, the activities of major antioxidant enzymes, catalase (CAT), glutathione S-transferase (GST), and superoxide dismutase (SOD), increased significantly, indicating enhanced tolerance to oxidative stress. Amplicon-based 16S rRNA gene sequencing revealed treatment-associated restructuring of the rhizosphere microbiome, with increased abundance of beneficial taxa, including Flavisolibacter, Azospirillum, and Novosphingobium. Metabolomic profiling further demonstrated the accumulation of stress-responsive metabolites, including DL-arginine, glycine betaine, oxaloacetic acid, 2,3-butanediol glucoside, and 5-aminolevulinic acid. Collectively, these findings demonstrate that the PGPR consortium enhances physiological resilience, reshapes rhizosphere microbial communities, and modulates plant metabolic responses under drought stress, demonstrating its applicability as an eco-friendly approach to increase rice yield under drought conditions.

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基于渗透耐受性- pgpr联合体的根际微生物组研究干旱胁迫下水稻代谢组学变化。

干旱是影响水稻（Oryza sativa L.）产量的关键限制因素，需要采取可持续的策略来提高作物在不断变化的气候条件下的抵御能力。研究了一种抗旱植物促生根瘤菌（PGPR）对水稻干旱胁迫的缓解作用，该菌株能够承受高达30% peg -6000诱导的渗透胁迫。联合体接种显著提高了干旱胁迫植株的相对含水量、脯氨酸积累和总叶绿素等关键生理指标。此外，过氧化氢酶（CAT）、谷胱甘肽s -转移酶（GST）和超氧化物歧化酶（SOD）等主要抗氧化酶活性显著升高，表明对氧化应激的耐受性增强。基于扩增子的16S rRNA基因测序揭示了根际微生物组与处理相关的重组，包括Flavisolibacter， Azospirillum和Novosphingobium等有益分类群的丰度增加。代谢组学分析进一步证明了应激反应代谢物的积累，包括dl -精氨酸、甘氨酸甜菜碱、草酰乙酸、2,3-丁二醇糖苷和5-氨基乙酰丙酸。综上所述，这些研究结果表明，PGPR联盟增强了干旱胁迫下的生理恢复力，重塑了根际微生物群落，调节了植物的代谢反应，证明了其作为干旱条件下水稻增产的生态友好方法的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.