Enhanced biodiesel production from Fusarium oxysporum via mutagenesis and process optimization using bagasse hydrolysate as a sustainable carbon source.

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2026-07-18 DOI:10.1007/s10529-026-03754-0

Sayeda Abdelrazek Abdelhamid, Einas Hamed El-Shatoury, Mohsen Selim Asker, Samir Khalef Abd-El-Aal, Ashraf Gamil Attallah, Sahar Saleh Mohamed, Mostafa Mostafa Abo Elsoud

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引用次数: 0

Abstract

Background: Biodiesel offers a sustainable alternative to fossil fuels with reduced greenhouse gas emissions; however, its commercial viability is constrained by high production costs. This study aimed to develop a cost-effective strategy for enhanced lipid and biodiesel production using Fusarium oxysporum NRC 2017, cultivated on sugarcane bagasse hydrolysate enzymatically saccharified by Bacillus cereus 3SME, as an inexpensive carbon source.

Methods: To improve lipid accumulation, the fungal strain was subjected to chemical and physical mutagenesis using gamma radiation (Ɣ-rays), ethidium bromide (EtBr), ethyl methane sulfonate (EMS), and sodium azide (NaN₃). Genetic variation between the wild type and induced mutants was confirmed by inter-simple sequence repeat (ISSR) analysis. The highest lipid-producing mutant, F. oxysporum NRC 2017-1, derived from gamma radiation, was selected for further optimization. Critical process parameters were optimized using response surface methodology (RSM) to maximize lipid yield.

Results: Under optimized conditions, the mutant strain achieved a maximum lipid yield of 3.81 g/L, representing a substantial increase over the wild type. Gas chromatography analysis revealed a favorable fatty acid methyl ester (FAME) profile, dominated by C16-C18 fatty acids, which meets the key compositional requirements for high-quality biodiesel. Furthermore, the physicochemical properties of the produced biodiesel, including cetane number, viscosity, and density, were within the recommended limits of international standards (ASTM D6751 and EN 14214).

Conclusion: This study presents an integrated and sustainable approach combining strain improvement via mutagenesis, valorization of lignocellulosic agricultural waste, and statistical process optimization. The findings highlight the potential of F. oxysporum NRC 2017-1 as a promising candidate for scalable, cost-effective biodiesel production.

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利用甘蔗渣水解物作为可持续碳源，通过诱变和工艺优化提高尖孢镰刀菌生产生物柴油的效率。

背景：生物柴油提供了化石燃料的可持续替代品，减少了温室气体排放；然而，其商业可行性受到高生产成本的限制。本研究旨在开发一种具有成本效益的策略，利用蜡样芽孢杆菌3SME在甘蔗甘蔗渣水解液上培养的镰刀菌NRC 2017作为廉价的碳源，提高脂质和生物柴油的产量。方法：为了改善脂质积累，对该真菌菌株进行了化学和物理诱变，包括伽马辐射（Ɣ-rays）、溴化乙酯（EtBr）、甲烷磺酸乙酯（EMS）和叠氮化钠（NaN₃）。通过ISSR分析证实了野生型和诱导型突变体之间存在遗传变异。我们选择了产脂率最高的突变体F. oxysporum NRC 2017-1进行进一步优化。利用响应面法（RSM）对关键工艺参数进行优化，使油脂得率最大化。结果：在优化条件下，突变菌株最大脂质产量为3.81 g/L，较野生型大幅提高。气相色谱分析显示，脂肪酸甲酯（FAME）以C16-C18脂肪酸为主，符合高品质生物柴油的关键成分要求。此外，所生产的生物柴油的物理化学性质，包括十六烷值、粘度和密度，都在国际标准（ASTM D6751和EN 14214）的推荐范围内。结论：本研究提出了一种综合的、可持续的方法，通过诱变、木质纤维素农业废弃物的增值和统计过程优化来改善菌株。这些发现突出了f.r oxysporum NRC 2017-1作为可扩展的、具有成本效益的生物柴油生产的有前途的候选物的潜力。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.