Exogenous recombinant complement proteins C3a and C5a regulate osteogenic differentiation of P-PDLSCs: a process associated with the Wnt/beta-catenin axis

IF 3.1 4区医学 Q3 IMMUNOLOGY

Immunobiology Pub Date : 2026-07-01 Epub Date: 2026-07-11 DOI:10.1016/j.imbio.2026.153218

Lingyan Peng, Yong Zhang, Xiaohui Yin, Fei Li, Jing Qiao

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引用次数: 0

Abstract

Objective

Concentrated growth factor (CGF) is rich in growth factors and complement proteins like C3a and C5a, showing promise for enhancing tissue regeneration. This study explores the regulatory effects of recombinant C3a/C5a proteins on the osteogenic differentiation of PDLSCs in an inflammatory microenvironment and their potential association with the Wnt/β-catenin pathway.

Methods

P-PDLSCs were generated by treating PDLSCs with IL-1β (5 ng/mL) and TNF-α (10 ng/mL) for 24 h. Subsequently, P-PDLSCs were treated with C5a and C3a, followed by evaluation of cell viability and early osteogenic differentiation capacity (ALP activity). To investigate the underlying mechanism, cells were treated with the Wnt/β-catenin pathway inhibitor, XAV939, either alone or combined with C3a/C5a. Cell viability, ALP activity, and the mRNA and protein levels of AKT, Runx2, and β-catenin were measured.

Results

Compared with the control group, exogenous recombinant C3a and C5a treatment can promote the proliferation and early osteogenic differentiation of P-PDLSCs. Additionally, through KEGG enrichment, the Wnt/β-catenin pathway was identified as a key target. The pro-osteogenic effects of C3a/C5a were partially reversed by XAV939, pointing to a potential correlation with the Wnt/β-catenin signaling pathway.

Conclusion

Exogenous recombinant C3a/C5a is associated with increased early osteogenic differentiation of P-PDLSCs, a process correlated with the activation of the Wnt/β-catenin signaling pathway, providing potential insights into regenerative strategies for periodontal tissues.

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外源性重组补体蛋白C3a和C5a调节P-PDLSCs的成骨分化：一个与Wnt/ β -连环蛋白轴相关的过程。

目的：浓缩生长因子（CGF）富含C3a、C5a等生长因子和补体蛋白，具有促进组织再生的潜力。本研究探讨了重组C3a/C5a蛋白对炎症微环境下PDLSCs成骨分化的调控作用及其与Wnt/β-catenin通路的潜在关联。方法：用IL-1β (5 ng/mL)和TNF-α (10 ng/mL)处理PDLSCs 24 h生成P-PDLSCs，然后用C5a和C3a处理P-PDLSCs，评估细胞活力和早期成骨分化能力（ALP活性）。为了研究潜在的机制，我们用Wnt/β-catenin通路抑制剂XAV939单独或与C3a/C5a联合处理细胞。测定细胞活力、ALP活性、AKT、Runx2、β-catenin mRNA和蛋白水平。结果：与对照组相比，外源性重组C3a和C5a处理能促进P-PDLSCs的增殖和早期成骨分化。此外，通过KEGG富集，Wnt/β-catenin通路被确定为关键靶点。C3a/C5a的促成骨作用被XAV939部分逆转，提示其可能与Wnt/β-catenin信号通路相关。结论：外源性重组C3a/C5a与P-PDLSCs早期成骨分化增加有关，这一过程与Wnt/β-catenin信号通路的激活有关，为牙周组织的再生策略提供了潜在的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.