Recombinant basic fibroblast growth factor produced in Nicotiana benthamiana enhances cell viability and wound healing.

IF 2.5 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2026-07-17 DOI:10.1007/s10529-026-03758-w

Ashwini Malla, Utapin Ngaokrajang, Kittinop Kittirotruji, Kittipong Sanookpan, Visarut Buranasudja, Balamurugan Shanmugaraj, Waranyoo Phoolcharoen

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Abstract

Basic Fibroblast Growth Factor (bFGF), also known as FGF2, is a multifunctional growth factor involved in several physiological and pathological processes, including angiogenesis, wound healing, and embryonic development. This study aimed to investigate the feasibility and efficacy of producing recombinant bFGF in Nicotiana benthamiana using a geminiviral transient expression vector. The primary objective was to assess the bioactivity of N. benthamiana-produced bFGF in comparison with commercially available recombinant bFGF preparations produced in different heterologous expression systems. NIH-3T3 cells were exposed to varying concentrations (0.5-1000 ng/mL) of plant-produced bFGF for 24 and 48 h. The assessment parameters included cell viability, cellular responses, and wound-healing capacity. The plant-produced bFGF exhibited slightly greater cell viability compared to the non-treated control and a modest increase in cell proliferation. The analysis of the dose-response curve yielded an EC50 value of 0.171 ng/mL highlighting the bioactivity and potency of plant-produced bFGF. These results demonstrate the efficacy of plant-derived bFGF in promoting cellular activities and facilitating wound closure, underscoring its potential for wound healing applications. However, further validation through preclinical and clinical studies is warranted.

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本菌烟草生产的重组碱性成纤维细胞生长因子提高细胞活力和伤口愈合。

碱性成纤维细胞生长因子（bFGF），也被称为FGF2，是一种多功能生长因子，参与多种生理和病理过程，包括血管生成、伤口愈合和胚胎发育。本研究旨在探讨利用双病毒瞬时表达载体在本烟中产生重组bFGF的可行性和有效性。主要目的是评估N. benthamiana生产的bFGF的生物活性，并与市售的不同异种表达系统生产的重组bFGF制剂进行比较。NIH-3T3细胞暴露于不同浓度（0.5-1000 ng/mL）的植物生成bFGF中24和48小时。评估参数包括细胞活力、细胞反应和伤口愈合能力。与未处理的对照相比，植物产生的bFGF表现出稍高的细胞活力和细胞增殖的适度增加。剂量响应曲线分析显示，EC50值为0.171 ng/mL，显示了植物源性bFGF的生物活性和效力。这些结果证明了植物源性bFGF在促进细胞活性和促进伤口愈合方面的功效，强调了其在伤口愈合应用中的潜力。然而，通过临床前和临床研究进一步验证是必要的。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.