Metabolic profiling of Polyalthia suberosa (Roxb.) Thwaites leaf via UPLC-ESI-MS/MS and highlighting its neuroprotective effect against scopolamine-induced memory impairment rat model

IF 3.1 Q2 MULTIDISCIPLINARY SCIENCES

Beni-Suef University Journal of Basic and Applied Sciences Pub Date : 2026-07-10 DOI:10.1186/s43088-026-00769-4

Orchid A. Mahmoud, Iriny M. Ayoub, Rasha A. Tawfiq, Omayma A. Eldahshan, Abdel Nasser B. Singab

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引用次数: 0

Abstract

Background

Alzheimer’s disease (AD) is an irrevocable neurodegenerative disorder that has caught global attention as the root cause of dementia. Polyalthia suberosa (Roxb.) Thwaites was reported to exhibit acetylcholinesterase inhibitory activity. To date, its potential protective effects against memory impairment have not been explored.

Methods

This study focused on evaluating the neuroprotective effect of Polyalthia suberosa leaf methanol extract (PSTE) and its dichloromethane soluble fraction (PSDF) against scopolamine (SCO)-induced memory impairment rat model. Additionally, metabolic profiling of PSTE and PSDF was carried out using Ultra-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry (UPLC-ESI-MS/MS). Anticholinesterase activity of PSTE and PSDF was evaluated in vitro. An in vivo study was conducted. Behavioral testing was performed using the elevated plus maze (EPM) and novel object recognition (NOR) tests. Additionally, histopathological examination, acetylcholinesterase activity, and levels were assessed.

Results

Results showed that both PSTE and PSDF displayed promising anticholinesterase activity in vitro, which was also confirmed by in vivo results where PSTE and PSDF at a dose 150 mg/kg were able to normalize the elevated acetylcholinesterase enzyme (AChE) level and activity in hippocampus tissue and minimized neurodegeneration in the brain tissue histopathologically assessed. The low dose of PSDF exhibited noticeable amelioration to SCO-induced memory deficit as evidenced by the significant elevation in the discrimination index and inflexion ratio in both NOR and EPM tests. Moreover, Metabolic profiling of PSTE and PSDF resulted in the tentative identification of 69 compounds, with alkaloids being the major class.

Conclusion

This unprecedented study highlights the substantial use of Polyalthia suberosa leaves as a natural neuroprotective adjuvant against AD.

Abstract Image

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蓼属植物（Polyalthia suberosa）的代谢谱分析通过UPLC-ESI-MS/MS验证其对东莨菪碱致记忆障碍大鼠模型的神经保护作用

阿尔茨海默病（AD）是一种不可逆转的神经退行性疾病，作为痴呆症的根本原因引起了全球的关注。长叶蓼（Roxb.）据报道，Thwaites具有乙酰胆碱酯酶抑制活性。迄今为止，它对记忆损伤的潜在保护作用尚未被探索。方法研究茯苓叶甲醇提取物（PSTE）及其二氯甲烷可溶组分（PSDF）对东莨菪碱（SCO）诱导的大鼠记忆损伤模型的神经保护作用。此外，使用超高效液相色谱-电喷雾电离质谱（UPLC-ESI-MS/MS）进行PSTE和PSDF的代谢谱分析。体外测定PSTE和PSDF抗胆碱酯酶活性。进行了体内研究。行为测试采用高架加迷宫（EPM）和新物体识别（NOR）测试。此外，组织病理学检查，乙酰胆碱酯酶活性和水平进行评估。结果表明，PSTE和PSDF在体外均显示出良好的抗胆碱酯酶活性，体内实验结果也证实了这一点，150 mg/kg剂量的PSTE和PSDF能够使海马组织中升高的乙酰胆碱酯酶（AChE）水平和活性正常化，并使脑组织组织病理学评估的神经变性最小化。低剂量PSDF对sco诱导的记忆缺陷有明显的改善作用，NOR和EPM试验的辨别指数和弯曲率均有显著升高。此外，对PSTE和PSDF进行代谢分析，初步鉴定出69个化合物，其中生物碱类为主要类别。结论：这一前所未有的研究表明，杜鹃花叶可作为抗阿尔茨海默病的天然神经保护佐剂。

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来源期刊

Beni-Suef University Journal of Basic and Applied Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

2.60

自引率

0.00%

发文量

期刊介绍： Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.