Karine R. Grigoryan, Ashkhen L. Zatikyan, Zara L. Grigoryan, Artur A. Gabrielyan, Hasmik A. Shilajyan
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引用次数: 0
Abstract
The effect of polystyrene nanoparticles (PSNPs) on binding interaction between Ganglefenum, an angionervalgic, anesthetic, and spasmolytic drug, and bovine serum albumin (BSA) was investigated using UV–Vis spectroscopy, fluorescence spectroscopy, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, and dynamic light scattering (DLS) techniques. UV–Vis spectroscopic analysis revealed that Ganglefenum forms a stable ground state complex with BSA, whereas fluorescence studies indicated the formation of comparatively less stable complexes, which may be attributed to competing quenching processes, conformational alterations, and non-radiative decay pathways affecting BSA fluorescence. Structural analysis based on deconvolution of the FTIR amide I band demonstrated significant changes in the secondary structure of BSA upon interaction with both Ganglefenum and PSNPs. DLS measurement showed the formation of larger complexes, as evidenced by changes in particle size distribution and diffusion coefficients for PSNPs, PS-bound BSA, and PS-bound BSA-Ganglefenum systems. The results of these studies suggest that PSNPs may interfere with the binding interactions between Ganglefenum and BSA.
期刊介绍：
The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size.
Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology.
The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.
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