{"title":"Potential of RadioHPLC for the Purification of [18F]anle138b, a Candidate Radiotracer for PET Diagnostics of Parkinson’s Disease","authors":"O. S. Fedorova, V. V. Orlovskaya, R. N. Krasikova","doi":"10.1134/S1066362226030124","DOIUrl":null,"url":null,"abstract":"<p>High radiochemical purity (RCP) is a crucial requirement to radiopharmaceuticals for positron emission tomography (PET). Semipreparative reversed-phase HPLC with UV and radioactivity detection is an efficient method for purifying radiopharmaceuticals. In separation of highly lipophilic molecules, most of the product remains on the chromatographic column owing to nonspecific sorption. We encountered this problem recently when developing a procedure for purifying [<sup>18</sup>F]anle138b, a candidate radiotracer for Parkinson’s disease diagnostics. In this study, we tested standard С18 columns, Supelco Ascentis RP-AMIDE, ACE 5 C18, and Macherey-Nagel Nucleosil, in the step gradient mode; however, the required RCP (>95%) was not reached. On the contrary, after the purification on a small monolithic Chromolith SemiPrep RP-18e column in the smooth gradient mode, RCP exceeded 98%. The procedure developed allows preparation of radiopharmaceuticals with the activity sufficient for preclinical studies.</p>","PeriodicalId":747,"journal":{"name":"Radiochemistry","volume":"68 3","pages":"388 - 397"},"PeriodicalIF":0.9000,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiochemistry","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1134/S1066362226030124","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
引用次数: 0
Abstract
High radiochemical purity (RCP) is a crucial requirement to radiopharmaceuticals for positron emission tomography (PET). Semipreparative reversed-phase HPLC with UV and radioactivity detection is an efficient method for purifying radiopharmaceuticals. In separation of highly lipophilic molecules, most of the product remains on the chromatographic column owing to nonspecific sorption. We encountered this problem recently when developing a procedure for purifying [18F]anle138b, a candidate radiotracer for Parkinson’s disease diagnostics. In this study, we tested standard С18 columns, Supelco Ascentis RP-AMIDE, ACE 5 C18, and Macherey-Nagel Nucleosil, in the step gradient mode; however, the required RCP (>95%) was not reached. On the contrary, after the purification on a small monolithic Chromolith SemiPrep RP-18e column in the smooth gradient mode, RCP exceeded 98%. The procedure developed allows preparation of radiopharmaceuticals with the activity sufficient for preclinical studies.
期刊介绍：
Radiochemistry is a journal that covers the theoretical and applied aspects of radiochemistry, including basic nuclear physical properties of radionuclides; chemistry of radioactive elements and their compounds; the occurrence and behavior of natural and artificial radionuclides in the environment; nuclear fuel cycle; radiochemical analysis methods and devices; production and isolation of radionuclides, synthesis of labeled compounds, new applications of radioactive tracers; radiochemical aspects of nuclear medicine; radiation chemistry and after-effects of nuclear transformations.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
