Development of fish scale collagen-immobilized poly(lactic acid) films using chemical treatment with hexamethylenediamine.

IF 1.4 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of oral science Pub Date : 2026-01-01 DOI:10.2334/josnusd.26-0015

Megumi Fuse

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引用次数: 0

Abstract

Purpose: This study aimed to evaluate how immobilizing tilapia fish scale type 1 collagen onto poly(lactic acid) (PLA), following chemical modification with hexamethylenediamine (HMDA), affects the adhesion and proliferation of mouse osteoblast-like cells (OLCs).

Methods: PLA films were chemically modified by HMDA immersion to introduce amino groups (-NH₂) (PLA-NH₂). Tilapia fish scale type 1 collagen was subsequently immobilized onto the PLA-NH₂ surface via a water-soluble carbodiimide condensation reaction, producing (TFColl-PLA-NH₂). Surface properties of the films were characterized using contact angle measurements and X-ray photoelectron spectroscopy (XPS). Biological evaluation was conducted by culturing OLCs on PLA, PLA-NH₂, and TFColl-PLA-NH₂ films, followed by quantification of cell numbers.

Results: The contact angles of PLA-NH₂ and TFColl-PLA-NH₂ were significantly lower than PLA (P < 0.05). XPS confirmed successful immobilization of tilapia collagen onto the PLA surface. At all time points, TFColl-PLA-NH₂ showed the highest cell numbers, followed by PLA-NH₂, while PLA had the lowest (P < 0.05). PLA-NH₂ promoted calcification more effectively than PLA (P < 0.05), and TFColl- PLA-NH₂ further enhanced calcification compared with PLA-NH₂ (P < 0.05).

Conclusion: PLA surface treatment with HMDA is an effective and straightforward method. Tilapia fish scale type 1 collagen has potential for application as a novel biomaterial source.

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用六亚二胺化学处理鱼鳞胶原-固定化聚乳酸薄膜的研制。

目的：研究罗非鱼鱼鳞1型胶原蛋白经六亚甲二胺（HMDA）化学修饰后，对小鼠成骨细胞样细胞（OLCs）粘附和增殖的影响。方法：采用HMDA浸渍法对聚乳酸薄膜进行化学修饰，引入氨基（-NH2）（PLA-NH2）。随后通过水溶性碳二亚胺缩合反应将罗非鱼鱼鳞1型胶原固定在PLA-NH2表面，生成（tfcol -PLA-NH2）。利用接触角测量和x射线光电子能谱（XPS）对膜的表面特性进行了表征。通过在PLA、PLA- nh2和tfcol -PLA- nh2薄膜上培养OLCs进行生物学评价，然后定量细胞数量。结果：PLA- nh2和tfcol -PLA- nh2的接触角显著低于PLA （P < 0.05）。XPS证实罗非鱼胶原蛋白成功固定在PLA表面。各时间点细胞数以tfcol -PLA- nh2最多，PLA- nh2次之，PLA最少（P < 0.05）。PLA- nh2比PLA更能促进钙化（P < 0.05）， TFColl- PLA- nh2比PLA- nh2更能促进钙化（P < 0.05）。结论：HMDA处理聚乳酸表面是一种简便有效的方法。罗非鱼鱼鳞1型胶原蛋白作为一种新型生物材料具有潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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