Phyto-synthesized silver nanoparticles from Dendrobium heyneanum and Coelogyne mossiae: a sustainable approach towards pharmaceutical applications.

IF 2.2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2026-08-01 Epub Date: 2026-07-14 DOI:10.1007/s10616-026-01028-1

Shivakrishnan Kaladharan, Sulaiman M, Meenaloshini Padmanaban, Subiya Showkat, Sivakumar Saroja Ramasubbu, Senthil Kumar Thiruppathi

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引用次数: 0

Abstract

Dendrobium heyneanum Lindl. and Coelogyne mossiae Rolfe, two critically conserved orchid species from the Western Ghats of India, remain among the least studied members of the Orchidaceae family due to their limited availability. In the present study, silver nanoparticles (AgNPs) were synthesized using aqueous extracts from in vitro-raised plants of D. heyneanum and C. mossiae, designated as DHSNP and CMSNP, respectively. The phyto-synthesized AgNPs were characterized using UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Dynamic light scattering (DLS), and Scanning electron microscopy (SEM). DHSNP and CMSNP exhibited UV peaks at 402 nm and 416 nm, respectively. FT-IR analysis identified functional groups such as alcohols, primary amines, aliphatic amines, and alkynes, while XRD confirmed their crystalline, Face-centered cubic structure. SEM revealed clustered particles with multiple-crystalline morphology. The phyto-synthesized AgNPs demonstrated notable antibacterial activity against Staphylococcus aureus (MTCC-7443), Bacillus subtilis (MTCC-7164) and Pseudomonas aeruginosa (MTCC-1688), with the highest zones of inhibition recorded against P. aeruginosa (27.59 mm for DHSNP and 26.00 mm for CMSNP). Both the phyto-synthesized AgNPs also exhibited promising antioxidant effects in the DPPH, Hydroxyl radical, Nitric oxide, and ABTS Scavenging assays. Furthermore, DHSNP reduced the cell viability of Human Glioblastoma U87-MG cells to 54.06%, whereas CMSNP reduced the viability of Human Melanoma SK-MEL-2 cells to 9.05%, accompanied by dose-dependent induction of apoptosis. Overall, the study demonstrates the antibacterial, antioxidant and anticancer activities of phyto-synthesized AgNPs from in vitro-raised D. heyneanum and C. mossiae, while establishing a sustainable approach for harnessing the pharmaceutical potential of unexplored orchid taxa.

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从石斛和苔藓中植物合成纳米银：一种可持续的药物应用方法。

石斛。和Coelogyne mossiae Rolfe，两种来自印度西高止山脉的高度保护的兰花物种，由于其有限的可用性，仍然是兰科家族中研究最少的成员之一。在本研究中，利用体外培养的D. heyneanum和C. mossiae植物（分别命名为DHSNP和CMSNP）的水提取物合成了银纳米粒子（AgNPs）。采用紫外可见光谱、傅里叶变换红外光谱（FT-IR）、x射线衍射（XRD）、动态光散射（DLS）和扫描电镜（SEM）对植物合成的AgNPs进行了表征。DHSNP和CMSNP分别在402 nm和416 nm处出现紫外峰。FT-IR分析确定了功能基团，如醇、伯胺、脂肪胺和炔，而XRD证实了它们的晶体、面心立方结构。扫描电镜观察到簇状颗粒具有多晶形态。植物合成的AgNPs对金黄色葡萄球菌（MTCC-7443）、枯草芽孢杆菌（MTCC-7164）和铜绿假单胞菌（MTCC-1688）具有显著的抑菌活性，其中对铜绿假单胞菌的抑制区最高（DHSNP为27.59 mm， CMSNP为26.00 mm）。这两种植物合成的AgNPs在DPPH、羟基自由基、一氧化氮和ABTS清除实验中也显示出良好的抗氧化作用。此外，DHSNP将人胶质母细胞瘤U87-MG细胞的细胞活力降低至54.06%，而CMSNP将人黑色素瘤SK-MEL-2细胞的细胞活力降低至9.05%，并伴有剂量依赖性诱导凋亡。总的来说，本研究证明了植物合成的AgNPs的抗菌、抗氧化和抗癌活性，同时为利用未开发的兰花类群的药用潜力建立了一个可持续的途径。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.