Viktoras Mazeika, Mykolas Maciulis, Kamdin Mirsanaye, Serguei Krouglov, Mehdi Alizadeh, Lukas Kontenis, Virginijus Barzda
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引用次数: 0
Abstract
Birefringence of collagen fibers influences second-harmonic generation (SHG) polarimetric measurements by introducing phase retardation for the incident fundamental beam and the SHG signal. The effect of birefringence on double Stokes polarimetry (DSP) parameters is investigated by imaging unstained rat tail tendon sections of varying thicknesses. In thin sections (≈10 μm thick), birefringence effects can be neglected; however, parameters combining linear and circular polarization states are affected, indicating a presence of complex-valued achiral susceptibility ratio due to fiber chirality. In thicker samples (≈80 μm thick), birefringence significantly affects DSP polarimetric and ultrastructural parameters, although approximate in-plane fiber orientation can still be retrieved. DSP polarimetric parameters enable rapid collagen characterization and are suited for large-area whole-slide investigations in histopathology.
期刊介绍：
The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including:
Tissue optics and spectroscopy
Novel microscopies
Optical coherence tomography
Diffuse and fluorescence tomography
Photoacoustic and multimodal imaging
Molecular imaging and therapies
Nanophotonic biosensing
Optical biophysics/photobiology
Microfluidic optical devices
Vision research.
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